WNT1 antibody (Internal Region)

Catalog No. ABIN964685

Supplier Product No.: 200-301-a37

The Mouse Monoclonal anti-WNT1 antibody (Clone 13F9) (ABIN964685) specifically detects WNT1 in WB and ELISA. The antibody is reactive with Mouse samples.

Quick Overview for WNT1 antibody (Internal Region) (ABIN964685)

Target: WNT1 (Wingless-Type MMTV Integration Site Family, Member 1 (WNT1))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This WNT1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: 13F9

Product Details anti-WNT1 Antibody

Binding Specificity: Internal Region

Supplier Product No.: 200-301-a37

Supplier: Rockland

Purpose: Wnt1 Antibody

Cross-Reactivity (Details): This antibody reacts with human and mouse Wnt1 protein.

Characteristics: Synonyms: mouse anti-WNT1 antibody, INT1, Proto-oncogene protein Wnt-1

Purification: Anti-Wnt1 antibody purified from tissue culture supernatant by Protein-A chromatography followed by extensive dialysis against the buffer stated above.

Sterility: Sterile filtered

Immunogen:

Immunogen: Synthetic peptide corresponding to an internal region of human Wnt1 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG1 kappa

Application Details

Application Notes:

Application Note: This protein-A purified antibody has been tested for use western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 41 kDa in size corresponding to Wnt1 by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:175 - 1:250

ELISA Dilution: 1:4,000 - 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for WNT1

Target: WNT1 (Wingless-Type MMTV Integration Site Family, Member 1 (WNT1))

Alternative Name: WNT1

Background: Background: The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt1 (Wingless-type MMTV integration site family member 1) is a member of the WNT gene family. It is highly conserved in evolution and the protein encoded by this gene is known to be 98 % identical to mouse Wnt1 protein at the amino acid level. Studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum. This gene was originally considered as a candidate gene for Joubert syndrome, an autosomal recessive disorder with cerebellar hypoplasia as a leading feature. However, further studies suggested that the gene mutations might not have a significant role in Joubert syndrome. Wnt1 is secreted as an extracellular matrix protein.

Gene ID: 7471, 139743

UniProt: P04628

Pathways: WNT Signaling, Dopaminergic Neurogenesis