IL-13 antibody

Catalog No. ABIN964711

Supplier Product No.: 200-401-b55

This Rabbit Polyclonal antibody specifically detects IL-13 in WB and ELISA. It exhibits reactivity toward Pig, Cow and Sheep.

Quick Overview for IL-13 antibody (ABIN964711)

Target: IL-13 (IL13) (Interleukin 13 (IL13))

Reactivity: Pig, Cow, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-13 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-IL-13 Antibody

Supplier Product No.: 200-401-b55

Supplier: Rockland

Purpose: IL13 Antibody

Cross-Reactivity (Details): This antibody is specific for swine IL-13 protein.

Characteristics: Synonyms: rabbit anti-Interleukin-13 antibody, rabbit anti-IL-13 antibody, IL13, Interleukin13, IL 13, Interleukin 13

Purification: This product was Protein-A purified from monospecific antiserum by chromatography.

Immunogen:

Immunogen: This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant protein raised in yeast, corresponding to mature swine IL-13 protein.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: IL-13 antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 13.2 kDa in size corresponding to swine IL-13 protein by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1.5 to 2 μg/mL

ELISA Dilution: 1:11,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for IL-13

Target: IL-13 (IL13) (Interleukin 13 (IL13))

Alternative Name: IL13

Background: Background: Interleukin 13 (IL-13), of the IL-4 superfamily, is a cytokine secreted by many cell types, but especially T helper type 2 (Th2) cells, that is an important mediator of allergic inflammation and disease. IL-13 induces its effects through a multi-subunit receptor that includes the alpha chain of the IL-4 receptor (IL-4Rα) and at least one IL-13-specific binding chain. Most of the biological effects of IL-13, like those of IL-4, are linked to a single transcription factor, STAT6. In humans, IL-13 can induce immunoglobulin E (IgE) secretion from activated B cells. In mice, deletion of IL-13 does not markedly affect either Th2 cell development or antigen-specific IgE responses induced by potent allergens. Deletion of IL-4 abrogates these responses. IL-13 acts as a molecular bridge linking allergic inflammatory cells to the non-immune cells in contact with them, thus altering physiological function. Although IL-13 is associated primarily with the induction of airway disease, including airway hyperresponsiveness, goblet cell metaplasia and mucus hypersecretion, it also induces airway matrix metalloproteinases as part of a mechanism that protects against excessive allergic inflammation that predisposes to asphyxiation. Anti-IL-13 antibody is ideal for investigators involved in Cancer and Immunology research.

Gene ID: 396721, 189176151

UniProt: B3GDZ5

Pathways: JAK-STAT Signaling, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport