IL-9 antibody

Catalog No. ABIN964759

Supplier Product No.: 209-401-b96

This Rabbit Polyclonal antibody specifically detects IL-9 in WB, IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for IL-9 antibody (ABIN964759)

Target: IL-9 (IL9) (Interleukin 9 (IL9))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-9 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-IL-9 Antibody

Supplier Product No.: 209-401-b96

Supplier: Rockland

Purpose: IL-9 Antibody

Characteristics: Synonyms: rabbit anti-IL-9 antibody, rabbit anti-interleukin-9 antibody, Interleukin 9, IL 9, IL9, T cell growth factor 3, T cell growth factor p40, TCGF 3, Mast cell growth factor, MCGF, Megakaryoblast growth factor, p40 cytokine

Purification: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This purified antibody has been heated to 56 °C for 30 minutes.

Immunogen:

Immunogen: This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-9 protein.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: User Optimized

Application Note: This purified antibody has been tested in western blotting. Reactivity is also expected in ELISA, neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/μl by the LAL method. By western blot a band approximately 15 kDa in size corresponding to native human IL-9 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1:1,000

ELISA Dilution: 1:10,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store Anti-IL-9 antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for IL-9

Target: IL-9 (IL9) (Interleukin 9 (IL9))

Alternative Name: IL9

Background: Background: IL-9 is a cytokine that acts as a regulator of a variety of hematopoietic cells. This cytokine stimulates cell proliferation and prevents apoptosis. It functions through the interleukin 9 receptor (IL9R), which activates different signal transducer and activator (STAT) proteins and thus connects this cytokine to various biological processes. The gene encoding this cytokine has been identified as a candidate gene for asthma. Genetic studies on a mouse model of asthma demonstrated that this cytokine is a determining factor in the pathogenesis of bronchial hyperresponsiveness. Anti-IL-9 antibody is ideal for investigators involved in Cancer and Immunology research.

Gene ID: 3578

NCBI Accession: NP_000581

UniProt: P15248

Pathways: JAK-STAT Signaling