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CCL4 antibody (Chemokine (C-C Motif) Ligand 4) Primary Antibody

CCL4 Reactivity: Human IHC, Neut, RIA, ELISA, WB Host: Rabbit Polyclonal
Catalog No. ABIN964760
$628.50
Plus shipping costs $45.00
100 μg
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  • Target
    Reactivity
    Human
    Host
    Rabbit
    Clonality
    Polyclonal
    Conjugate
    This CCL4 antibody is un-conjugated
    Application
    Immunohistochemistry (IHC), Neutralization (Neut), Radioimmunoassay (RIA), ELISA, Western Blotting (WB)
    Specificity
    This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL-9 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
    Characteristics
    MIP1 alpha and MIP1 beta were originally co-purified from medium conditioned by an LPS-stimulated murine macrophage cell line. Human MIP1 beta refers to the products of several independently cloned cDNAs, including Act2, PAT 744, hH400, G26, HIMAP, HC21, and MAD 5a. The predicted protein products of these cDNAs represent variants that are between 94% - 98% identical and these proteins are all approximately 75% homologous to murine MIP1 beta. MIP1 beta also shares approximately 70% amino acid identity with MIP1 alpha. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. The MIP1 proteins have chemoattractant and adhesive effects on lymphocytes, with MIP1 alpha and MIP1 beta preferentially attracting CD8+ and CD4+ T cells, respectively. A signal transducing receptor designated the CC chemokine receptor 1 (CC CKR1) with seven transmembrane domains that binds MIP1 alpha, MIP1 beta, MCP1 and RANTES with varying affinities has been isolated.
    Purification
    purified
    Immunogen
    This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human MIP-1ß protein.
    Immunogen Type: RecombinantProtein
    Isotype
    IgG
  • Application Notes
    This purified antibody has been tested for use in ELISA and western blotting. Reactivity is also expected in neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/µl by the LAL method. By western blot a band approximately 15 kDa in size corresponding to native human MIP-1ß protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
    Comment

    Gene Name: CCL4

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL
    Concentration
    1.0 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Preservative
    Without preservative
    Storage
    4 °C/-20 °C
    Storage Comment
    Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
    Expiry Date
    12 months
  • Target
    Alternative Name
    MIP-1 beta (CCL4 Antibody Abstract)
    Synonyms
    ACT2, AT744.1, G-26, HC21, LAG-1, LAG1, MIP-1-beta, MIP1B, MIP1B1, SCYA2, SCYA4, CCL4, K203, Mip1-b, Scya4, MIP-1beta, MIP-1BETA, MIP1b, SCY4A, Act-2, MIP-1B, Mip1b, C-C motif chemokine ligand 4, chemokine (C-C motif) ligand 4, chemokine (C-C motif) ligand 4-like 1, CCL4, Ccl4, CCL4L1
    Background
    MIP1 alpha and MIP1 beta were originally co-purified from medium conditioned by an LPS-stimulated murine macrophage cell line. Human MIP1 beta refers to the products of several independently cloned cDNAs, including Act2, PAT 744, hH400, G26, HIMAP, HC21, and MAD 5a. The predicted protein products of these cDNAs represent variants that are between 94% - 98% identical and these proteins are all approximately 75% homologous to murine MIP1 beta. MIP1 beta also shares approximately 70% amino acid identity with MIP1 alpha. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. The MIP1 proteins have chemoattractant and adhesive effects on lymphocytes, with MIP1 alpha and MIP1 beta preferentially attracting CD8+ and CD4+ T cells, respectively. A signal transducing receptor designated the CC chemokine receptor 1 (CC CKR1) with seven transmembrane domains that binds MIP1 alpha, MIP1 beta, MCP1 and RANTES with varying affinities has been isolated.
    Synonyms: CCL4, C-C motif chemokine 4, Small-inducible cytokine A4, Macrophage inflammatory protein 1-beta, MIP-1-beta, ACT-2, T-cell activation protein 2, Protein H400, Lymphocyte activation gene 1 protein, LAG-1, HC21, G-26 T-lymphocyte-secreted protein, MIP-1ß
    Gene ID
    6351
    NCBI Accession
    NP_002975
    UniProt
    P13236
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