AKT1 antibody

Catalog No. ABIN965526

Product Details anti-AKT1 Antibody

Target: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AKT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Specificity: Akt (Ab-473) Antibody detects endogenous levels of total Akt protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: This antiserum was produced against synthesized non-phosphopeptide derived from human Akt around the phosphorylation site of serine 473 (Q-F-SP-Y-S).

Application Details

Application Notes: WB: 1:500~1:1000
IHC: 1:50~1:100.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100ug/100ul.

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-AKT1 antibody (ABIN965526)

Baudhuin, Jiang, Zaslavsky, Ishii, Chun, Xu: "S1P3-mediated Akt activation and cross-talk with platelet-derived growth factor receptor (PDGFR)." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 18, Issue 2, pp. 341-3, (2004) (PubMed).

Feng, Tamaskovic, Yang, Brazil, Merlo, Hess, Hemmings: "Stabilization of Mdm2 via decreased ubiquitination is mediated by protein kinase B/Akt-dependent phosphorylation." in: The Journal of biological chemistry, Vol. 279, Issue 34, pp. 35510-7, (2004) (PubMed).

Ayala, Thompson, Yang, Frolov, Li, Scardino, Ohori, Wheeler, Harper: "High levels of phosphorylated form of Akt-1 in prostate cancer and non-neoplastic prostate tissues are strong predictors of biochemical recurrence." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 10, Issue 19, pp. 6572-8, (2004) (PubMed).

Lungu, Jin, Yamawaki, Tanimoto, Wong, Berk: "Cyclosporin A inhibits flow-mediated activation of endothelial nitric-oxide synthase by altering cholesterol content in caveolae." in: The Journal of biological chemistry, Vol. 279, Issue 47, pp. 48794-800, (2004) (PubMed).

Target Details for AKT1

Target: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Alternative Name: Akt (AKT1 Products)

Pathways: PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling, Warburg Effect