PLAP antibody

Catalog No. ABIN965542

This Mouse Monoclonal antibody specifically detects PLAP in WB, IHC and ELISA. It exhibits reactivity toward Human. It has been mentioned in 2+ publications

Quick Overview for PLAP antibody (ABIN965542)

Target: PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PLAP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: 3E5

Product Details anti-PLAP Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human ALPP expressed in E. Coli.

Isotype: IgG2b

Application Details

Application Notes: Western Bloting: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Handling

Format: Liquid

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-PLAP antibody (ABIN965542)

Llinas, Stura, Ménez, Kiss, Stigbrand, Millán, Le Du: "Structural studies of human placental alkaline phosphatase in complex with functional ligands." in: Journal of molecular biology, Vol. 350, Issue 3, pp. 441-51, (2005) (PubMed).

Chen, Anderson, Hiroi, Medof: "Proprotein interaction with the GPI transamidase." in: Journal of cellular biochemistry, Vol. 88, Issue 5, pp. 1025-37, (2003) (PubMed).

Target Details for PLAP

Target: PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))

Alternative Name: ALPP

Background: There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placentallike form. The coding sequence for this form of alkaline phosphatase is unique in that the 3' untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized.
Synonyms: ALP, PALP, PLAP, FLJ61142, ALPP

Molecular Weight: 58kDa

Gene ID: 250