Blood Group H antibody

Catalog No. ABIN965679

The Mouse Monoclonal anti-Blood Group H antibody (Clone B389) (ABIN965679) specifically detects Blood Group H in IHC (p), IHC (fro) and IHC (fp). The antibody is reactive with Human samples.

Quick Overview for Blood Group H antibody (ABIN965679)

Target: Blood Group H

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: B389

Product Details

Specificity: This antibody is specific for epitope Fuca1-2 Gal and detects blood group antigens H, H2, Ley and Leb. There is no cross-reactivity with blood group antigens A, B, Lea and Lex. The specificity of this antibody closely resembles that of MAb clone M/A 15-5.

Purification: Concentrated.

Immunogen: Neuraminidase-treated live cells of the T-47D breast carcinoma cell lines.

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry: 1:50 - 1:100.
Staining Procedure: This antibody can be used on formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:50 - 1:100 in IHC. It is recommended that this product be used on frozen tissue sections or specimens. The optimal conditions should be determined by the individual laboratory.

Comment:

Recommended Positive Control: Brain

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.3 mg/ml.

Buffer: 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details

Target: Blood Group H

Gene ID: 2523