BTK antibody

Catalog No. ABIN965707

This Monoclonal antibody specifically detects BTK in WB, ELISA and IHC. It exhibits reactivity toward Human and has been mentioned in 3+ publications.

Quick Overview for BTK antibody (ABIN965707)

Target: BTK (Bruton Agammaglobulinemia tyrosine Kinase (BTK))

Reactivity: Human

Host: Please inquire

Clonality: Monoclonal

Conjugate: This BTK antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-BTK Antibody

Specificity: Ni-NTA purified truncated recombinant BTK expressed in E. Coli strain BL21 (DE3)

Purification: Crude ascites.

Isotype: IgG1

Application Details

Application Notes: Western Blot: 1: 500- 1: 2,000
IHC(P): 1: 500- 1: 2,000
ELISA: Propose dilution 1: 10,000.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: -20 °C

References for anti-BTK antibody (ABIN965707)

Tamagnone, Lahtinen, Mustonen, Virtaneva, Francis, Muscatelli, Alitalo, Smith, Larsson, Alitalo: "BMX, a novel nonreceptor tyrosine kinase gene of the BTK/ITK/TEC/TXK family located in chromosome Xp22.2." in: Oncogene, Vol. 9, Issue 12, pp. 3683-8, (1994) (PubMed).

Yamada, Kawakami, Kimura, Fukamachi, Baier, Altman, Kato, Inagaki, Kawakami: "Structure and expression of novel protein-tyrosine kinases, Emb and Emt, in hematopoietic cells." in: Biochemical and biophysical research communications, Vol. 192, Issue 1, pp. 231-40, (1993) (PubMed).

Thomas, Sideras, Smith, Vorechovský, Chapman, Paul: "Colocalization of X-linked agammaglobulinemia and X-linked immunodeficiency genes." in: Science (New York, N.Y.), Vol. 261, Issue 5119, pp. 355-8, (1993) (PubMed).

Target Details for BTK

Target: BTK (Bruton Agammaglobulinemia tyrosine Kinase (BTK))

Alternative Name: BTK

Background: Brutons tyrosine kinase (BTK) is a member of the BTK/ Tec family of cytoplasmic tyrosine kinases.All members of the family contain SH3 and SH2 domains and, with the exception of Txk and Dsrc28C, also contain a pleckstrin homology (PH) and a Tec homology (TH) domain in their amino termini.BTK plays an important role in B cell development. Activation of B cells by various ligands is accompanied by BTK membrane translocation mediated by its PH domain binding to phosphatidylinositol-3,4,5trisphosphate. The membrane located BTK is active and associated with transient phosphorylation of two tyrosine residues, Tyr551 and Tyr223. Tyr551 in the activation loop is transphosphorylated by the Src family tyrosine kinase, leading to autophosphorylation at Tyr223 within the SH3 domain, which is necessary for full activation.

Gene ID: 695

Pathways: Fc-epsilon Receptor Signaling Pathway, Hormone Transport, Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, BCR Signaling