CD44 antibody

Catalog No. ABIN965799

The Mouse Monoclonal anti-CD44 antibody (Clone B313 (DF1485)) (ABIN965799) specifically detects CD44 in FACS, IF and IHC (fro). The antibody is reactive with Human samples.

Quick Overview for CD44 antibody (ABIN965799)

Target: CD44

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD44 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: B313 (DF1485)

Product Details anti-CD44 Antibody

Specificity: This antibody reacts with CD44, a glycoprotein of varying molecular weight (29-37 kD on hematopoietic cells and 51 kD on epithelial cells).

Purification: Purified.

Isotype: IgG1 kappa

Application Details

Application Notes: Immunofluorescence: 5-20 ug/mL.
Flow cytometry: 1-5 ug/10^6 cells.
Immunoblotting: 1-5 ug/mL.
Immunohistochemistry: 5-20 ug/mL on frozen sections using avidin-biotin system.
This product was not quality controlled in flow cytometry.
Staining Procedure: This antibody can be used on acetone fixed frozen cryostat sections as well as formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation is buffered formalin can destroy the epitope. The antibody may be used at a dilution of 5-20ug/ml in IHC. For formalin-fixed paraffin-embedded tissue sections, high temperature antigenic unmasking techniques is required. The optimal condition should be determinded by individual laboratory.

Comment:

Recommended Positive Control: Tonsil and Lymph Node

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.4 mg/ml.

Buffer: 50 mM Sodium Borate, 150 mM Sodium Chloride, 20% Glycerol, pH 8.0

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for CD44

Target: CD44

Alternative Name: CD44,Phagocytic Glycoprotein-1

Gene ID: 960

Pathways: Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling