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CD44 antibody

CD44 Reactivity: Human FACS, IF, IHC (fro) Host: Mouse Monoclonal B313 (DF1485) unconjugated

Catalog No. ABIN965799

Datasheet as PDF

Target See all CD44 Antibodies

CD44
Reactivity
297

135

71

31

25

23

14

13

10

9

8

7

7

5

5

4

2

2

1

1

1

1

1

1
Human
Host
178

119

74

1

1

1
Mouse
Compatible Secondaries
Clonality
261

110

2
Monoclonal
Conjugate
164

42

31

22

16

7

6

6

5

5

5

4

4

3

3

3

3

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1
This CD44 antibody is un-conjugated
Application
277

147

85

71

70

66

51

41

40

30

26

15

7

6

5

5

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity

This antibody reacts with CD44, a glycoprotein of varying molecular weight (29-37 kD on hematopoietic cells and 51 kD on epithelial cells).

Purification

Purified.

Clone

B313 (DF1485)

Isotype

IgG1 kappa

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CD44 Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

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CD44 Reactivity: Human, Mouse, Rat WB, IHC, ELISA Host: Rabbit Polyclonal unconjugated

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CD44 Reactivity: Human WB, IHC (p) Host: Mouse Polyclonal unconjugated

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Application Notes

Immunofluorescence: 5-20 ug/mL.
Flow cytometry: 1-5 ug/10^6 cells.
Immunoblotting: 1-5 ug/mL.
Immunohistochemistry: 5-20 ug/mL on frozen sections using avidin-biotin system.
This product was not quality controlled in flow cytometry.
Staining Procedure: This antibody can be used on acetone fixed frozen cryostat sections as well as formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation is buffered formalin can destroy the epitope. The antibody may be used at a dilution of 5-20ug/ml in IHC. For formalin-fixed paraffin-embedded tissue sections, high temperature antigenic unmasking techniques is required. The optimal condition should be determinded by individual laboratory.

Comment

Recommended Positive Control: Tonsil and Lymph Node

Restrictions

For Research Use only
Format

Liquid

Concentration

0.4 mg/ml.

Buffer

50 mM Sodium Borate, 150 mM Sodium Chloride, 20% Glycerol, pH 8.0

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C
Target

CD44

Alternative Name

CD44,Phagocytic Glycoprotein-1 (CD44 Products)

Gene ID

960

Pathways

Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling