Cytokeratin 19 antibody (AA 312-335)

Catalog No. ABIN965960

The Mouse Monoclonal anti-Cytokeratin 19 antibody has been validated for WB, IHC (p) and IHC (fro). It is suitable to detect Cytokeratin 19 in samples from Human, Mouse and Rat.

Quick Overview for Cytokeratin 19 antibody (AA 312-335) (ABIN965960)

Target: Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 19 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: B303 (A53-B-A2)

Product Details anti-Cytokeratin 19 Antibody

Binding Specificity: AA 312-335

Purification: Concentrated.

Immunogen: Keratin fraction isolated from Human Breast Cancer MCF-7 cells

Isotype: IgG2a

Application Details

Application Notes: Immunohistochemistry: (Paraffin/Frozen): 1:50-1:100
WB: 1:100-1:500.
Staining Procedure: Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:50 to 1:100 with. The optimal conditions should be determined by the individual laboratory. High temperature antigen unmasking technique predigestion is strongly recommended.

Comment:

Cellular Localization: Cytoplasmic.
Recommended Positive Control: Skin, Colonic Carcinoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.3 mg/ml

Buffer: 20 mM Tris-Borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing Fetal Bovine Serum, BMC-6 Carrier Polysaccharides, Carrier Protein, pH 7.5

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for Cytokeratin 19

Target: Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

Background: This antibody reacts with Cytokeratin 19 (40 kD) intermediate filament and with a wide variety of Simple epithelia and non-squamous stratified epithelia. It does not react with stratified squamous epithelia. However, cervical squamous cell carcinomas show positivity with this antibody.

Gene ID: 3880