MUC1 antibody (DTRP (Asp-Thr-Arg-Pro))

Catalog No. ABIN966067

This Mouse Monoclonal antibody specifically detects MUC1 in IHC (p) and IHC (fro). It exhibits reactivity toward Human and has been mentioned in 2+ publications.

Quick Overview for MUC1 antibody (DTRP (Asp-Thr-Arg-Pro)) (ABIN966067)

Target: MUC1 (Mucin 1 (MUC1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MUC1 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: B24-1 (GP1-4)

Product Details anti-MUC1 Antibody

Binding Specificity: DTRP (Asp-Thr-Arg-Pro)

Purification: Concentrated.

Immunogen: Human Milk Fat Globule Membranes

Isotype: IgG1 kappa

Application Details

Application Notes: Immunohistochemistry: (Paraffin/Frozen): 1:50.
Staining Procedure: Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:50 in IHC. It is recommended that this product be used on frozen tissue sections or specimens. The optimal conditions should be determined by the individual laboratory.

Comment:

Cellular Localization: Cell Membrane, Cytoplasmic.
Recommended Positive Control: Breast Carcinoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.3 mg/ml.

Buffer: 20 mM Tris-Borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing Fetal Bovine Serum, BMC-6 Carrier Polysaccharides, Carrier Protein, pH 7.5

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-MUC1 antibody (ABIN966067)

Hilkens, Ligtenberg, Vos, Litvinov: "Cell membrane-associated mucins and their adhesion-modulating property." in: Trends in biochemical sciences, Vol. 17, Issue 9, pp. 359-63, (1992) (PubMed).

Hilkens, Buijs, Hilgers, Hageman, Calafat, Sonnenberg, van der Valk: "Monoclonal antibodies against human milk-fat globule membranes detecting differentiation antigens of the mammary gland and its tumors." in: International journal of cancer. Journal international du cancer, Vol. 34, Issue 2, pp. 197-206, (1984) (PubMed).

Target Details for MUC1

Target: MUC1 (Mucin 1 (MUC1))

Alternative Name: EMA

Background: Reacts with human high molecular weight sialomucin, episialin, mucin 1 (MUC-1), or polymorphic epithelial mucin (PEM). It recognizes the multiple protein epitope that is relatively insensitive to glycosylation. Therefore, it is very sensitive in the detection of episialin expression in different epithelial tissues.

Molecular Weight: 265-400 kDa

Gene ID: 4582

Pathways: Negative Regulation of intrinsic apoptotic Signaling