GFP antibody (Green Fluorescent Protein)

Details for Product anti-GFP Antibody No. ABIN966200
Antigen
Reactivity
Aequorea victoria
401
10
10
9
9
7
6
3
2
1
Host
Mouse
168
155
59
29
16
7
3
2
Clonality (Clone)
Monoclonal ()
Conjugate
This GFP antibody is un-conjugated
19
16
12
9
6
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), ELISA, Western Blotting (WB)
344
196
107
87
83
54
27
25
23
20
15
12
10
8
8
7
6
4
3
1
1
1
Options
Immunogen Purified recombinant fragment of GFP expressed in E. Coli.
Clone 4B10B2
Isotype IgG2a
Purification Purified
Antigen
Alternative Name GFP
Background GFP (Green fluorescence protein) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light when excited by blue light. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors. GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.
Molecular Weight 27kDa
Application Notes Western Bloting: 1/500 - 1/2000.
Immunofluorescence: 1/200 - 1/1000.
Flow cytometry: 1/200 - 1/400.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Buffer PBS
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Product cited in: Dittman, Kaplan: "Factors regulating the abundance and localization of synaptobrevin in the plasma membrane." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 30, pp. 11399-404, 2006 (PubMed).

Cavalieri, Bernardo, Spinelli: "Regulatory sequences driving expression of the sea urchin Otp homeobox gene in oral ectoderm cells." in: Gene expression patterns : GEP, Vol. 7, Issue 1-2, pp. 124-30, 2006 (PubMed).