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GFP antibody (Green Fluorescent Protein) Primary Antibody

GFP Reactivity: Aequorea victoria FACS, IF, ELISA, WB Host: Mouse Monoclonal 4B10B2 unconjugated
Pubmed (2)
Catalog No. ABIN966200
$462.00
Plus shipping costs $45.00
0.1 mg
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  • Target
    GFP
    Reactivity
    Aequorea victoria
    Host
    Mouse
    Clonality
    Monoclonal
    Conjugate
    This GFP antibody is un-conjugated
    Application
    Flow Cytometry (FACS), Immunofluorescence (IF), ELISA, Western Blotting (WB)
    Purification
    Purified
    Immunogen
    Purified recombinant fragment of GFP expressed in E. Coli.
    Clone
    4B10B2
    Isotype
    IgG2a
  • Application Notes
    Western Bloting: 1/500 - 1/2000.
    Immunofluorescence: 1/200 - 1/1000.
    Flow cytometry: 1/200 - 1/400.
    ELISA: Propose dilution 1/10000.
    Not yet tested in other applications.
    Determining optimal working dilutions by titration test.
    Restrictions
    For Research Use only
  • Buffer
    PBS
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
  • Dittman, Kaplan: "Factors regulating the abundance and localization of synaptobrevin in the plasma membrane." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 30, pp. 11399-404, 2006 (PubMed).

    Cavalieri, Bernardo, Spinelli: "Regulatory sequences driving expression of the sea urchin Otp homeobox gene in oral ectoderm cells." in: Gene expression patterns : GEP, Vol. 7, Issue 1-2, pp. 124-30, 2006 (PubMed).

  • Target
    GFP
    Alternative Name
    GFP
    Synonyms
    green fluorescent protein, gfp
    Background
    GFP (Green fluorescence protein) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light when excited by blue light. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors. GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.
    Molecular Weight
    27kDa
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