ErbB2/Her2 antibody

Catalog No. ABIN966277

This Rabbit Polyclonal antibody specifically detects ErbB2/Her2 in IHC. It exhibits reactivity toward Human. It has been mentioned in 5+ publications

Quick Overview for ErbB2/Her2 antibody (ABIN966277)

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ErbB2/Her2 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Product Details anti-ErbB2/Her2 Antibody

Specificity: HER2 (Ab-1221/1222) antibody detects endogenous levels of total HER2 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human HER2 around the phosphorylation site of tyrosine 1221/1222 (N-L-YP-YP -W).

Application Details

Application Notes: WB: 1:500~1:1000
IHC: 1:50~1:100.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100ug/100ul.

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-ErbB2/Her2 antibody (ABIN966277)

Marone, Hess, Dankort, Muller, Hynes, Badache: "Memo mediates ErbB2-driven cell motility." in: Nature cell biology, Vol. 6, Issue 6, pp. 515-22, (2004) (PubMed).

Ren, Schaefer: "ErbB-2 activates Stat3 alpha in a Src- and JAK2-dependent manner." in: The Journal of biological chemistry, Vol. 277, Issue 41, pp. 38486-93, (2002) (PubMed).

Klapper, Waterman, Sela, Yarden: "Tumor-inhibitory antibodies to HER-2/ErbB-2 may act by recruiting c-Cbl and enhancing ubiquitination of HER-2." in: Cancer research, Vol. 60, Issue 13, pp. 3384-8, (2000) (PubMed).

Ouyang, Gulliford, Epstein: "The duration of phorbol-inducible ErbB2 tyrosine dephosphorylation parallels that of receptor endocytosis rather than threonine-686 phosphorylation: implications for the physiological role of protein kinase C in growth factor receptor signalling." in: Carcinogenesis, Vol. 19, Issue 11, pp. 2013-9, (1998) (PubMed).

Ouyang, Huang, Chantry, Epstein: "Adjacent carboxyterminal tyrosine phosphorylation events identify functionally distinct ErbB2 receptor subsets: implications for molecular diagnostics." in: Experimental cell research, Vol. 241, Issue 2, pp. 467-75, (1998) (PubMed).

Target Details for ErbB2/Her2

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Alternative Name: HER2

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development