CCL2 antibody (N-Term)

Catalog No. ABIN966546

Product Details anti-CCL2 Antibody

Target: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCL2 antibody is un-conjugated

Application: Western Blotting (WB)

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for C-C motif chemokine 2 ( CCL2) detection. Tested with WB in Human, Mouse, Rat.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminal of human MCP-1, different from the mouse and rat sequence by two amino acids< br/>Immunogen was affinity purified.

Isotype: IgG

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Sodium azide, Thimerosal (Merthiolate)

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: At -20 °C for one year. After reconstitution, at 4 °C for one month. It can also be aliquotted and stored frozen at -20 °C for a longer time.

References for anti-CCL2 antibody (ABIN966546)

Gosling, Slaymaker, Gu, Tseng, Zlot, Young, Rollins, Charo: "MCP-1 deficiency reduces susceptibility to atherosclerosis in mice that overexpress human apolipoprotein B." in: The Journal of clinical investigation, Vol. 103, Issue 6, pp. 773-8, (1999) (PubMed).

Gu, Okada, Clinton, Gerard, Sukhova, Libby, Rollins: "Absence of monocyte chemoattractant protein-1 reduces atherosclerosis in low density lipoprotein receptor-deficient mice." in: Molecular cell, Vol. 2, Issue 2, pp. 275-81, (1998) (PubMed).

Furutani, Nomura, Notake, Oyamada, Fukui, Yamada, Larsen, Oppenheim, Matsushima: "Cloning and sequencing of the cDNA for human monocyte chemotactic and activating factor (MCAF)." in: Biochemical and biophysical research communications, Vol. 159, Issue 1, pp. 249-55, (1989) (PubMed).

Target Details for CCL2

Target: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Alternative Name: C-C motif chemokine 2 (CCL2 Products)

Synonyms: GDCF-2 antibody, HC11 antibody, HSMCR30 antibody, MCAF antibody, MCP-1 antibody, MCP1 antibody, SCYA2 antibody, SMC-CF antibody, AI323594 antibody, JE antibody, Scya2 antibody, Sigje antibody, MCP-1A antibody, MCP1A antibody, C-C motif chemokine ligand 2 antibody, chemokine (C-C motif) ligand 2 antibody, C-C motif chemokine 2 antibody, CCL2 antibody, Ccl2 antibody, LOC101120093 antibody

Background: Monocyte chemoattractant protein-1 (MCP-1), a member of the chemokine (chemotactic cytokine) family, is a potent monocyte agonist that is upregulated by oxidized lipids. 1 MCP-1 is also known as CCL2, SCYA2, MCAF. MCAF is a member of family of factors involved in immune and inflammatory responses. The amino acid sequence deduced from the nucleotide sequence reveals the primary structure of the MCAF precursor to be composed of a putative signal peptide sequence of 23 amino acid residues and a mature MCAF sequence of 76 amino acid residues. 2 MCP-1 plays a unique and crucial role in the initiation of atherosclerosis and may provide a new therapeutic target in this disorder. 3 Human MCP-1 is a 8. 7KDa non-glycoprotein, consisting of 99 amino acids in precursor form and 76 amino acids in mature form.

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, The Global Phosphorylation Landscape of SARS-CoV-2 Infection