NUMA1 antibody (Cytoplasmic, Nuclear)

Catalog No. ABIN966706

Product Details anti-NUMA1 Antibody

Target: NUMA1 (Nuclear Mitotic Apparatus Protein 1 (NUMA1))

Binding Specificity: Cytoplasmic, Nuclear

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NUMA1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Reacts with an intracellular 228 kDa protein found inthe nucleus during interphase.

Purification: Concentrated.

Immunogen: Live Ls 174T cells (colon carcinoma).

Clone: 3-3A

Isotype: IgM

Application Details

Application Notes: Immunohistochemistry: 1:50-1:100 on frozen and formalin-fixed paraffin-embedded tissue sections. (For paraffin sections high temp. antigen demasker, 10mM citrate, pH 6.0, is required, (boiling tissue for 10 mins, followed by cooling for 10-20 mins).
Western Blot 1:100-1:300.
Staining Procedure: This antibody can be used on frozen and formalin tissue sections. Formalin-fixed paraffin-embdded tissue sections require, boilng the tisssue in 10 mM citrate buffer, pH 6.0 for 10-20 mins. followed by cooling to Rt for 10-20 mins. This step is done prior to antibody. The antibody may be used at a dilution of 1:50-1:100 in IHC. The optimal conditions should be determined by the individual laboratory.

Comment:

Recommended Positive Control: Tonsil, Thymus, Spleen

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/ml.

Buffer: 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-NUMA1 antibody (ABIN966706)

Gueth-Hallonet, Weber, Osborn: "Cleavage of the nuclear matrix protein NuMA during apoptosis." in: Experimental cell research, Vol. 233, Issue 1, pp. 21-4, (1997) (PubMed).

Target Details for NUMA1

Target: NUMA1 (Nuclear Mitotic Apparatus Protein 1 (NUMA1))

Alternative Name: NuMA (NUMA1 Products)

Molecular Weight: 228 kDa

Gene ID: 4926

Pathways: Caspase Cascade in Apoptosis, Regulation of Actin Filament Polymerization, M Phase