SCPA (C-Term) antibody

Catalog No. ABIN967004

There are 5+ publications for this product available. The Rabbit Polyclonal anti-SCPA antibody is suitable to detect SCPA in samples from Staphylococcus aureus. It has been validated for IHC.

Quick Overview for SCPA (C-Term) antibody (ABIN967004)

Target: SCPA

Reactivity: Staphylococcus aureus

Host: Rabbit

Clonality: Polyclonal

Application: Immunohistochemistry (IHC)

Product Details

Binding Specificity: C-Term

Immunogen: Polyclonal antibody produced in rabbits immunizing with a synthetic peptide corresponding to C-terminal residues of Bacteria: Staphylococcus aureus scpA (Staphylococcal cysteine proteinaseA)

Application Details

Restrictions: For Research Use only

Handling

Storage: 4 °C

References

Rodrigues Lopes, Alcantara, Silva, Josse, Vega, Cabrerizo, Bonhomme, Lopez, Laurent, Vandenesch, Mano, Eulalio: "Microscopy-based phenotypic profiling of infection by Staphylococcus aureus clinical isolates reveals intracellular lifestyle as a prevalent feature." in: Nature communications, Vol. 13, Issue 1, pp. 7174, (2022) (PubMed).

Stelzner, Boyny, Hertlein, Sroka, Moldovan, Paprotka, Kessie, Mehling, Potempa, Ohlsen, Fraunholz, Rudel: "Intracellular Staphylococcus aureus employs the cysteine protease staphopain A to induce host cell death in epithelial cells." in: PLoS pathogens, Vol. 17, Issue 9, pp. e1009874, (2021) (PubMed).

Golonka, Filipek, Sabat, Sinczak, Potempa: "Genetic characterization of staphopain genes in Staphylococcus aureus." in: Biological chemistry, Vol. 385, Issue 11, pp. 1059-67, (2004) (PubMed).

Dubin, Krajewski, Popowicz, Stec-Niemczyk, Bochtler, Potempa, Dubin, Holak: "A novel class of cysteine protease inhibitors: solution structure of staphostatin A from Staphylococcus aureus." in: Biochemistry, Vol. 42, Issue 46, pp. 13449-56, (2003) (PubMed).

Potempa, Dubin, Korzus, Travis: "Degradation of elastin by a cysteine proteinase from Staphylococcus aureus." in: The Journal of biological chemistry, Vol. 263, Issue 6, pp. 2664-7, (1988) (PubMed).

Target Details

Target: SCPA

Background: The scpA (Staphylococcal cysteine proteinaseA) is able to degrade elastin. scpA (Staphylococcal cysteine proteinaseA) has a broad endopeptidase action on proteins including elastin, but rather limited hydrolysis of small-molecule substrates. Assays are conveniently made with hemoglobin, casein or Z-Phe-Arg-NHMec as substrate. Prematurely activated/folded staphopain A is inhibited by staphostatin A (scpB), which is probably required to protect staphylococcal cytoplasmic proteins from degradation by scpA. In the cytoplasm, prematurely activated/folded scpA forms a stable non-covalent complex with scpB. Cleavage leads to the activation of scpA probably by an auto-catalytic manner. The catalytic maturation of scpA appears to reside outside the cascade of activation started by the metalloprotease aureolysin (aur) scpA (Staphylococcal cysteine proteinaseA) belongs to the peptidase C47 family.
Synonyms: sspP, sspB2, Staphopain A