Desmin antibody

Catalog No. ABIN967294

This Mouse Monoclonal antibody specifically detects Desmin in WB and IF. It exhibits reactivity toward Human, Rat, Mouse and Chicken and has been mentioned in 4+ publications.

Quick Overview for Desmin antibody (ABIN967294)

Target: Desmin (DES)

Reactivity: Human, Rat, Mouse, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This Desmin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: RD301

Product Details anti-Desmin Antibody

Brand: BD Pharmingen™

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Human

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Purified desmin from chicken gizzard

Isotype: IgG2b

Application Details

Application Notes: Applications include western blot analysis and immunofluorescence microscopy on frozen sections although IF is not tested. Mouse muscle lysate is recommended as a positive control.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-Desmin antibody (ABIN967294)

Schaart, Viebahn, Langmann, Ramaekers: "Desmin and titin expression in early postimplantation mouse embryos." in: Development (Cambridge, England), Vol. 107, Issue 3, pp. 585-96, (1990) (PubMed).

Raats, Pieper, Vree Egberts, Verrijp, Ramaekers, Bloemendal: "Assembly of amino-terminally deleted desmin in vimentin-free cells." in: The Journal of cell biology, Vol. 111, Issue 5 Pt 1, pp. 1971-85, (1990) (PubMed).

Pieper, Schaart, Krimpenfort, Henderik, Moshage, van de Kemp, Ramaekers, Berns, Bloemendal: "Transgenic expression of the muscle-specific intermediate filament protein desmin in nonmuscle cells." in: The Journal of cell biology, Vol. 108, Issue 3, pp. 1009-24, (1989) (PubMed).

Broers, Carney, Klein Rot, Schaart, Lane, Vooijs, Ramaekers: "Intermediate filament proteins in classic and variant types of small cell lung carcinoma cell lines: a biochemical and immunochemical analysis using a panel of monoclonal and polyclonal antibodies." in: Journal of cell science, Vol. 83, Issue 12, pp. 37-60, (1987) (PubMed).

Target Details for Desmin

Target: Desmin (DES)

Alternative Name: Desmin

Background: Intermediate filaments (IF) are a subset of cytoskeletal proteins which function to give overall structural integrity to the plasma membrane as well as organize cells into specific tissues. IF proteins can be divided into six major types based upon the similarity in sequence. Desmin belongs to the type III category of IF proteins which are predominantly expressed in muscle cells including cardiac, skeletal and smooth muscle. Furthermore, the expression of desmin is regulated in a stage and tissue-specific manner, since it is induced during terminal differentiation of skeletal muscle cells. In skeletal cardiac muscle cells, desmin is localized in the Z-disk region and at the intercalated disk and acts to stabilize sarcomeres in stimulated muscle. Desmin migrates in SDS/PAGE as a 53 kDa protein.

Molecular Weight: 53 kDa