PARP1 antibody

Catalog No. ABIN967302

This anti-PARP1 antibody is a Mouse Monoclonal antibody detecting PARP1 in WB, IP, FACS and Func. Suitable for Human. This Primary Antibody has been cited in 2+ publications.

Quick Overview for PARP1 antibody (ABIN967302)

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PARP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS), Functional Studies (Func)

Clone: 7D3-6

Product Details anti-PARP1 Antibody

Brand: BD Pharmingen™

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Purified human PARP

Isotype: IgG1

Application Details

Application Notes: Jurkat control lysate [50 µg (1 µg/µl)] is provided as a western blot positive control (store lysate at -20°C). Additional control lysates (ABIN968537 and ABIN967299) are sold separately. Western blot analysis of PARP. Control (lanes 1-3) and treated Jurkat lysates (lanes 4-6) were probed with anti-PARP (clone 7D3-6) at concentrations of 2.0 (lanes 1, 4), 1.0 (lanes 2, 5), and 0.5 µg/ml (lanes 3, 6). Intact PARP is identified as a band of 116 kDa, and cleaved PARP as a band of ~85 kDa.

Comment:

Related Products: ABIN968537, ABIN967299

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-PARP1 antibody (ABIN967302)

Ranjit, Cheng, Mackay, Whitacre, Berger, Berger: "Poly(adenosine diphosphoribose) polymerase in peripheral blood leukocytes from normal donors and patients with malignancies." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 1, Issue 2, pp. 223-34, (1999) (PubMed).

Patel, Gores, Kaufmann: "The role of proteases during apoptosis." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 10, Issue 5, pp. 587-97, (1996) (PubMed).

Target Details for PARP1

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Alternative Name: PARP

Background: PARP [Poly(ADP-ribose) polymerase] is a 116 kDa nuclear chromatin-associated enzyme that catalyzes the transfer of ADP-ribose units from NAD+ to a variety of nuclear proteins including topoisomerases, histones, and PARP itself. The catalytic activity of PARP is increased in nonapoptotic cells following DNA damage, and PARP is thought to play an important role in mediating the normal cellular response to DNA damage. Additionally, PARP is a target of the caspase protease activity associated with apoptosis. During apoptosis, PARP is cleaved from the 116 kDa intact form into 85 kDa and 25 kDa fragments. This process separates the amino-terminal DNA-binding domain of the enzyme from the carboxy-terminal catalytic domain resulting in the loss of normal PARP function. Although the role of PARP in apoptosis remains to be elucidated, PARP cleavage is considered to be a marker of apoptosis. PARP antibodies [Clone 4C10-5 (ABIN967540), Clone 7D3-6, and Clone C2-10 (ABIN967515)] all recognize both the intact 116 kDa form and 85 kDa fragment of PARP. The 7D3-6 antibody recognizes both the 116 kDa intact form and the 85 kDa fragment of human PARP. It recognizes both native and denatured PARP. It reacts with an epitope located in the NAD binding domain. Purified human PARP was used as immunogen. The antibody was originally characterized by western blot analysis, flow cytometric analysis, and by dot blot assays. In dot blot assays, the antibody reacts with the native enzyme in the presence or absence of bound DNA as well as after synthesis of covalently linked poly(ADP-ribose).

Molecular Weight: 116 kDa (full-length), 85 kDa (cleaved)

Pathways: Apoptosis, Caspase Cascade in Apoptosis, DNA Damage Repair, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location