Clusterin antibody

Catalog No. ABIN967369

Product Details anti-Clusterin Antibody

Target: Clusterin (CLU)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Clusterin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Brand: BD Pharmingen™

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Clone: E5

Isotype: IgG1 kappa

Application Details

Application Notes: Immunohistochemistry:
The E5 antibody, specific for human clusterin, is tested for immunohistochemical staining of acetone-fixed frozen sections and zinc-fixed and formalin-fixed, paraffin-embedded sections. Tissue tested was human tonsil. The antibody stains dendritic cells in the follicle and basement membrane of epithelial cells. The isotype control recommended for use with this antibody is purified mouse IgG1. For optimal indirect immunohistochemical staining, the E5 antibody should be titrated (1:10 to 1:50 dilution) and visualized via a three-step staining procedure in combination with polyclonal, biotin conjugated anti-mouse Igs (multiple adsorbed) as the secondary antibody and Streptavidin-HRP together with the DAB Detection System. More conveniently, the Anti-Mouse Ig HRP Detection Kit, which contains the biotinylated secondary antibody, antibody diluent, Streptavidin-HRP and DAB substrate, can be used for staining.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-Clusterin antibody (ABIN967369)

Wellmann, Thieblemont, Pittaluga, Sakai, Jaffe, Siebert, Raffeld: "Detection of differentially expressed genes in lymphomas using cDNA arrays: identification of clusterin as a new diagnostic marker for anaplastic large-cell lymphomas." in: Blood, Vol. 96, Issue 2, pp. 398-404, (2000) (PubMed).

Rosenberg, Silkensen: "Clusterin: physiologic and pathophysiologic considerations." in: The international journal of biochemistry & cell biology, Vol. 27, Issue 7, pp. 633-45, (1995) (PubMed).

Murphy, Kirszbaum, Walker, dApice: "SP-40,40, a newly identified normal human serum protein found in the SC5b-9 complex of complement and in the immune deposits in glomerulonephritis." in: The Journal of clinical investigation, Vol. 81, Issue 6, pp. 1858-64, (1988) (PubMed).

Target Details for Clusterin

Target: Clusterin (CLU)

Alternative Name: Clusterin (CLU Products)

Synonyms: LOC100136120 antibody, clu antibody, MGC53055 antibody, zgc:73352 antibody, xclu antibody, MGC89163 antibody, CLU antibody, APO-J antibody, APOJ antibody, CLI antibody, CLU1 antibody, CLU2 antibody, KUB1 antibody, NA1/NA2 antibody, SGP-2 antibody, SGP2 antibody, SP-40 antibody, TRPM-2 antibody, TRPM2 antibody, DAG antibody, RATTRPM2B antibody, SP40 antibody, TRPM2B antibody, Trpm2 antibody, Trpmb antibody, AI893575 antibody, ApoJ antibody, Cli antibody, D14Ucla3 antibody, Sgp-2 antibody, Sgp2 antibody, Sugp-2 antibody, GP80 antibody, gpIII antibody, 40 antibody, clusterin-1 antibody, clusterin L homeolog antibody, clusterin antibody, Clusterin antibody, LOC100136120 antibody, clu.L antibody, clu antibody, CLU antibody, clus antibody, Clu antibody, LOC101113728 antibody

Background: The E5 antibody reacts with human clusterin. Clusterin was originally isolated and characterized from glomerular immune deposits of patients with renal glomerulonephritis. Clusterin, an 80 kD disulfide-linked, heterodimeric glycoprotein (previously named SP-40,40 [serum protein 40 kD,40kD]) was shown to be part of the SC5b-9 complex of complement, yet separate and distinct from the S protein. Clusterins have since been identified in a number of mammalian species and have been implicated in diverse cellular activities, however, the exact role remains to be elucidated.

Pathways: Apoptosis, Negative Regulation of intrinsic apoptotic Signaling