Integrin beta 4 antibody

Catalog No. ABIN967380

Product Details anti-Integrin beta 4 Antibody

Target: Integrin beta 4 (ITGB4)

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Integrin beta 4 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Brand: BD Pharmingen™

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Sodium azide is a reversible inhibitor of oxidative metabolism, therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Tumor-associated antigen TSP-180 from a BALB/c mouse lung carcinoma

Clone: 346-11A

Isotype: IgG2a kappa

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4° C.

References for anti-Integrin beta 4 antibody (ABIN967380)

Morena, Riccioni, Marchetti, Polcini, Mercurio, Blandino, Sacchi, Falcioni: "Expression of the beta 4 integrin subunit induces monocytic differentiation of 32D/v-Abl cells." in: Blood, Vol. 100, Issue 1, pp. 96-106, (2002) (PubMed).

Giancotti: "Signal transduction by the alpha 6 beta 4 integrin: charting the path between laminin binding and nuclear events." in: Journal of cell science, Vol. 109 ( Pt 6), pp. 1165-72, (1997) (PubMed).

van der Neut, Krimpenfort, Calafat, Niessen, Sonnenberg: "Epithelial detachment due to absence of hemidesmosomes in integrin beta 4 null mice." in: Nature genetics, Vol. 13, Issue 3, pp. 366-9, (1996) (PubMed).

Sonnenberg, Calafat, Janssen, Daams, van der Raaij-Helmer, Falcioni, Kennel, Aplin, Baker, Loizidou: "Integrin alpha 6/beta 4 complex is located in hemidesmosomes, suggesting a major role in epidermal cell-basement membrane adhesion." in: The Journal of cell biology, Vol. 113, Issue 4, pp. 907-17, (1991) (PubMed).

Sonnenberg, Linders, Daams, Kennel: "The alpha 6 beta 1 (VLA-6) and alpha 6 beta 4 protein complexes: tissue distribution and biochemical properties." in: Journal of cell science, Vol. 96 ( Pt 2), pp. 207-17, (1990) (PubMed).

Kennel, Foote, Falcioni, Sonnenberg, Stringer, Crouse, Hemler: "Analysis of the tumor-associated antigen TSP-180. Identity with alpha 6-beta 4 in the integrin superfamily." in: The Journal of biological chemistry, Vol. 264, Issue 26, pp. 15515-21, (1989) (PubMed).

Kennel, Foote, Flynn: "Tumor antigen on benign adenomas and on murine lung carcinomas quantitated by a two-site monoclonal antibody assay." in: Cancer research, Vol. 46, Issue 2, pp. 707-12, (1986) (PubMed).

Target Details for Integrin beta 4

Target: Integrin beta 4 (ITGB4)

Alternative Name: CD104 (ITGB4 Products)

Synonyms: AA407042 antibody, C230078O20 antibody, CD104 antibody, integrin subunit beta 4 antibody, integrin beta 4 antibody, ITGB4 antibody, Itgb4 antibody

Background: The 346-11A antibody reacts with an epitope at the beginning of the cysteine-rich repeat region of the 200-kDa integrin beta4 chain (CD104), which is found on the cell surface as a heterodimeric complex with the integrin alpha6 chain (CD49f). The alpha6beta4 complex binds to laminins and is expressed on the basal surface of a variety of epithelial cell types, particularly on stratified squamous epithelia, and is also found in peripheral nerves, in certain subsets of endothelial cells, and on immature thymocytes. It has also been identified on a number of tumor tissues and participates in tumor progression events. Because of the localization of both human and mouse epidermal alpha6beta4 integrin to hemidesmosomes, a role in epidermal adhesion to basement membrane has been suggested.
Synonyms: Integrin beta4 chain

Pathways: Integrin Complex