Panendothelial Cell antibody

Catalog No. ABIN967385

There are 6+ publications for this product available. The Rat Monoclonal anti-Panendothelial Cell antibody is suitable to detect Panendothelial Cell in samples from Mouse. It has been validated for IHC (fro), FACS, IP and WB.

Quick Overview for Panendothelial Cell antibody (ABIN967385)

Target: Panendothelial Cell

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)

Clone: MECA-32

Product Details

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse lymph node stromal cells

Isotype: IgG2a kappa

Application Details

Application Notes: For IHC, we recommend the use of purified MECA-32 mAb in our special formulation for immunohistochemistry.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4° C.

References

Hallmann, Mayer, Berg, Broermann, Butcher: "Novel mouse endothelial cell surface marker is suppressed during differentiation of the blood brain barrier." in: Developmental dynamics : an official publication of the American Association of Anatomists, Vol. 202, Issue 4, pp. 325-32, (1995) (PubMed).

Bergese, Pelletier, Ohye, Vallera, Orosz: "Treatment of mice with anti-CD3 mAb induces endothelial vascular cell adhesion molecule-1 expression." in: Transplantation, Vol. 57, Issue 5, pp. 711-7, (1994) (PubMed).

Engelhardt, Conley, Butcher: "Cell adhesion molecules on vessels during inflammation in the mouse central nervous system." in: Journal of neuroimmunology, Vol. 51, Issue 2, pp. 199-208, (1994) (PubMed).

Penn, Jiang, Fei, Sitnicka, Wolf: "Dissecting the hematopoietic microenvironment. IX. Further characterization of murine bone marrow stromal cells." in: Blood, Vol. 81, Issue 5, pp. 1205-13, (1993) (PubMed).

Orosz, van Buskirk, Sedmak, Kincade, Miyake, Pelletier: "Role of the endothelial adhesion molecule VCAM in murine cardiac allograft rejection." in: Immunology letters, Vol. 32, Issue 1, pp. 7-12, (1992) (PubMed).

Leppink, Bishop, Sedmak, Henry, Ferguson, Streeter, Butcher, Orosz: "Inducible expression of an endothelial cell antigen on murine myocardial vasculature in association with interstitial cellular infiltration." in: Transplantation, Vol. 48, Issue 5, pp. 874-7, (1989) (PubMed).

Target Details

Target: Panendothelial Cell

Background: The MECA-32 antibody reacts with a dimer of 50-55-kDa subunits expressed on most or all endothelial cells in the embryonic and adult mouse, with the exception of cardiac and skeletal muscle and the brain. Normally in skeletal and cardiac muscle, MECA-32 antigen expression is limited to small arterioles and venules, however, under conditions of inflammation, it can be induced on previously non-expressing vessels in cardiac muscle. In the central nervous system (CNS), the panendothelial cell antigen expression is developmentally regulated. During embryonic development, the antigen is found on brain vasculature up to day 16 of gestation, after which it disappears. The cessation of MECA-32 antigen expression in the CNS may be associated with the establishment of the blood-brain barrier, which begins on day 16 of gestation. In the adult mouse, inflammation in the CNS can lead to re-expression of the panendothelial cell antigen.