Dendritic Cells antibody

Details for Product No. ABIN967479, Supplier: Log in to see
Rat (Rattus)
Clonality (Clone)
Monoclonal ()
Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
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Supplier Product No.
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Brand BD Pharmingen™
Immunogen Density gradient-enriched PVG rat thoracic-duct dendritic cells
Clone OX-62
Isotype IgG1, kappa
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Sodium azide is a reversible inhibitor of oxidative metabolism, therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Background The OX-62 antibody reacts with an antigen on dendritic cells, dendritic epidermal T cells (but not RT1B+ Langerhans cells), and intestinal intraepithelial lymphocytes of normal rats and on CD3+ lymphocytes in the spleen and cervical lymph nodes of athymic nude rats. The antigen can be detected by flow cytometric or immunocytochemical analysis of leukocytes and by immunohistochemical staining of epidermal sheets, wholemounts of neural tissue, and frozen sections of lymphoid and non-lymphoid organs. The OX-62 mAb immunoprecipitates a molecule with integrin-like properties, and sequencing indicates that it is related to mouse and human CD103 (Integrin alphaIEL or alphaE chain).
Research Area Cell/Tissue Markers
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.
Supplier Images
Flow Cytometry (FACS) image for anti-Dendritic Cells antibody (ABIN967479) Immunofluorescent staining and flow cytometric analysis of enriched dendritic cells f...
Flow Cytometry (FACS) image for anti-Dendritic Cells antibody (ABIN967479) Immunofluorescent staining and flow cytometric analysis of enriched dendritic cells f...
Background publications McMenamin: "Distribution and phenotype of dendritic cells and resident tissue macrophages in the dura mater, leptomeninges, and choroid plexus of the rat brain as demonstrated in wholemount preparations." in: The Journal of comparative neurology, Vol. 405, Issue 4, pp. 553-62, 1999 (PubMed).

Brenan, Rees: "Sequence analysis of rat integrin alpha E1 and alpha E2 subunits: tissue expression reveals phenotypic similarities between intraepithelial lymphocytes and dendritic cells in lymph." in: European journal of immunology, Vol. 27, Issue 11, pp. 3070-9, 1998 (PubMed).

Nelson, McMenamin, McWilliam, Brenan, Holt: "Development of the airway intraepithelial dendritic cell network in the rat from class II major histocompatibility (Ia)-negative precursors: differential regulation of Ia expression at different levels of the respiratory tract." in: The Journal of experimental medicine, Vol. 179, Issue 1, pp. 203-12, 1994 (PubMed).

Product cited in: Penfield, Wang, Li, Kielar, Sicher, Jeyarajah, Lu: "Transplant surgery injury recruits recipient MHC class II-positive leukocytes into the kidney." in: Kidney international, Vol. 56, Issue 5, pp. 1759-69, 1999 (PubMed).

Gieseler, Hoffmann, Kuhn, Fayyazi, Stojanovic, Schlemminger, Peters: "Enrichment and characterization of dendritic cells from rat renal mesangium." in: Scandinavian journal of immunology, Vol. 46, Issue 6, pp. 587-96, 1998 (PubMed).

Chen-Woan, Delaney, Fournier, Wakizaka, Murase, Fung, Starzl, Demetris: "In vitro characterization of rat bone marrow-derived dendritic cells and their precursors." in: Journal of leukocyte biology, Vol. 59, Issue 2, pp. 196-207, 1996 (PubMed).

Brenan, Puklavec: "The MRC OX-62 antigen: a useful marker in the purification of rat veiled cells with the biochemical properties of an integrin." in: The Journal of experimental medicine, Vol. 175, Issue 6, pp. 1457-65, 1992 (PubMed).

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