Phosphotyrosine antibody (HRP)

Catalog No. ABIN967694

There are 5+ publications for this product available. The Mouse Monoclonal anti-Phosphotyrosine antibody is suitable to detect Phosphotyrosine. It has been validated for WB, IP, IF and IHC.

Quick Overview for Phosphotyrosine antibody (HRP) (ABIN967694)

Target: Phosphotyrosine

Host: Mouse

Clonality: Monoclonal

Conjugate: This Phosphotyrosine antibody is conjugated to HRP

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC)

Clone: PY20

Product Details anti-Phosphotyrosine Antibody (HRP)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Isotype: IgG2b

Application Details

Application Notes: The use of milk-containing buffers may interfere with a phosphotyrosine antibody's ability to bind specific proteins of interest. Please use BSA-containing buffers for blocking and incubating purposes. This antibody is routinely tested by western blot analysis. Other applications were tested during antibody development only or reported in the literature.

Comment:

Related Products: ABIN968534

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol.

Storage: -20 °C

Storage Comment: The antibody was conjugated with HRP under optimum conditions, and unconjugated antibody and free HRP were removed by gel filtration chromatography. Store undiluted at -20° C.

References for anti-Phosphotyrosine antibody (HRP) (ABIN967694)

Hemmeryckx, Reichert, Watanabe, Kaartinen, de Jong, Pattengale, Groffen, Heisterkamp: "BCR/ABL P190 transgenic mice develop leukemia in the absence of Crkl." in: Oncogene, Vol. 21, Issue 20, pp. 3225-31, (2002) (PubMed).

Alderton, Rakhit, Kong, Palmer, Sambi, Pyne, Pyne: "Tethering of the platelet-derived growth factor beta receptor to G-protein-coupled receptors. A novel platform for integrative signaling by these receptor classes in mammalian cells." in: The Journal of biological chemistry, Vol. 276, Issue 30, pp. 28578-85, (2001) (PubMed).

Borges, Jan, Ruoslahti: "Platelet-derived growth factor receptor beta and vascular endothelial growth factor receptor 2 bind to the beta 3 integrin through its extracellular domain." in: The Journal of biological chemistry, Vol. 275, Issue 51, pp. 39867-73, (2001) (PubMed).

Goitsuka, Tatsuno, Ishiai, Kurosaki, Kitamura: "MIST functions through distinct domains in immunoreceptor signaling in the presence and absence of LAT." in: The Journal of biological chemistry, Vol. 276, Issue 38, pp. 36043-50, (2001) (PubMed).

Smith, Surviladze, Gaudet, Backer, Mitchell, Wilson: "p110beta and p110delta phosphatidylinositol 3-kinases up-regulate Fc(epsilon)RI-activated Ca2+ influx by enhancing inositol 1,4,5-trisphosphate production." in: The Journal of biological chemistry, Vol. 276, Issue 20, pp. 17213-20, (2001) (PubMed).

Target Details for Phosphotyrosine

Target: Phosphotyrosine

Target Type: Amino Acid

Background: Phosphorylation of specific tyrosine residues is the result of of activation or stimulation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be autophosphorylated kinases or certain cellular protein substrates that are regulated in oncogenesis or cell growth. Antibodies to phosphotyrosine provide one of the best tools for the detection and characterization of phosphotyrosine proteins. Technical Note: The use of milk-containing buffers may interfere with a phosphotyrosine antibody's ability to bind specific proteins of interest. Please use BSA-containing buffers for blocking and incubating purposes. This antibody is routinely tested by western blot analysis.