RAP2A antibody (AA 1-183)

Catalog No. ABIN967831

This Mouse Monoclonal antibody specifically detects RAP2A in WB, IF, IHC (f) and IP. It exhibits reactivity toward Human, Mouse, Rat, Dog, Chicken and Frog and has been mentioned in 5+ publications.

Quick Overview for RAP2A antibody (AA 1-183) (ABIN967831)

Target: RAP2A (RAP2A, Member of RAS Oncogene Family (RAP2A))

Reactivity: Human, Mouse, Rat, Dog, Chicken, Frog

Host: Mouse

Clonality: Monoclonal

Conjugate: This RAP2A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP)

Clone: 12-Rap2

Product Details anti-RAP2A Antibody

Binding Specificity: AA 1-183

Cross-Reactivity: Chicken, Dog (Canine), Frog, Mouse (Murine), Rat (Rattus)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Rap2 aa. 1-183

Isotype: IgG2a

Application Details

Comment:

Related Products: ABIN968533, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-RAP2A antibody (ABIN967831)

Ohba, Mochizuki, Matsuo, Yamashita, Nakaya, Hashimoto, Hamaguchi, Kurata, Nagashima, Matsuda: "Rap2 as a slowly responding molecular switch in the Rap1 signaling cascade." in: Molecular and cellular biology, Vol. 20, Issue 16, pp. 6074-83, (2000) (PubMed).

Reedquist, Bos: "Costimulation through CD28 suppresses T cell receptor-dependent activation of the Ras-like small GTPase Rap1 in human T lymphocytes." in: The Journal of biological chemistry, Vol. 273, Issue 9, pp. 4944-9, (1998) (PubMed).

Janoueix-Lerosey, Polakis, Tavitian, de Gunzburg: "Regulation of the GTPase activity of the ras-related rap2 protein." in: Biochemical and biophysical research communications, Vol. 189, Issue 1, pp. 455-64, (1992) (PubMed).

Ohmstede, Farrell, Reep, Clemetson, Lapetina: "RAP2B: a RAS-related GTP-binding protein from platelets." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 17, pp. 6527-31, (1990) (PubMed).

Pizon, Chardin, Lerosey, Olofsson, Tavitian: "Human cDNAs rap1 and rap2 homologous to the Drosophila gene Dras3 encode proteins closely related to ras in the 'effector' region." in: Oncogene, Vol. 3, Issue 2, pp. 201-4, (1988) (PubMed).

Target Details for RAP2A

Target: RAP2A (RAP2A, Member of RAS Oncogene Family (RAP2A))

Alternative Name: Rap2

Background: Rap2 is a member of the Ras superfamily of low molecular weight GTP/GDP binding proteins. The Rap proteins are 50% homologous in sequence to p21ras. Like Ras, the Rap proteins cycle between a GDP-bound inactive form and a GTP-bound active form. Conversion between these two forms is regulated by Rap-GTPase activating protein (Rap-GAP) and a Rap-GDP dissociation stimulator (GDS). Since Ras and Rap have the same amino acid sequence in their putative effector domain (aa. 32-40), it seems likely that they perform either similar or antagonistic functions. Rap2 proteins are about 60% identical to Rap1 proteins and Rap2A and Rap2B show 90% amino acid identity, differing mainly at the carboxy-terminus. Unlike Rap1, the Rap2 proteins cannot compete with Ras for interaction with Ras-GAP, nor are they substrates for PKA. It follows that the intrinsic GTPase activity of Rap2A is not stimulated by Ras-GAP, however a distinct activator (Rap-GAP) has been identified. Both Rap2 proteins show posttranslational modifications: Rap2B is geranylgeranylated and Rap2A is the first non-Ras member of the Ras superfamily observed to be farnesylated. This antibody is routinely tested by western blot analysis.

Molecular Weight: 21 kDa