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NOS1 antibody (AA 1095-1289)

NOS1 Reactivity: Human, Rat, Mouse, Dog WB, IHC, IF, IP Host: Mouse Monoclonal 16-nNOS-NOS Type I unconjugated
Catalog No. ABIN967895
  • Target See all NOS1 Antibodies
    NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))
    Binding Specificity
    • 27
    • 15
    • 12
    • 9
    • 6
    • 6
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 1095-1289
    Reactivity
    • 127
    • 89
    • 62
    • 15
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Rat, Mouse, Dog
    Host
    • 134
    • 20
    • 3
    Mouse
    Clonality
    • 128
    • 29
    Monoclonal
    Conjugate
    • 69
    • 20
    • 11
    • 6
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This NOS1 antibody is un-conjugated
    Application
    • 117
    • 61
    • 47
    • 31
    • 28
    • 27
    • 26
    • 20
    • 18
    • 12
    • 8
    • 6
    • 5
    • 4
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
    Cross-Reactivity
    Rat (Rattus), Mouse (Murine), Dog (Canine)
    Characteristics
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Human nNOS aa. 1095-1289
    Clone
    16-nNOS-NOS Type I
    Isotype
    IgG2a
    Top Product
    Discover our top product NOS1 Primary Antibody
  • Comment

    Related Products: ABIN967389, ABIN968545

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    250 μg/mL
    Buffer
    Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store undiluted at -20° C.
  • Schuh, Uldrijan, Telkamp, Rothlein, Neyses: "The plasmamembrane calmodulin-dependent calcium pump: a major regulator of nitric oxide synthase I." in: The Journal of cell biology, Vol. 155, Issue 2, pp. 201-5, (2001) (PubMed).

    Sasaki, Gonzalez-Zulueta, Huang, Herring, Ahn, Ginty, Dawson, Dawson: "Dynamic regulation of neuronal NO synthase transcription by calcium influx through a CREB family transcription factor-dependent mechanism." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 15, pp. 8617-22, (2000) (PubMed).

    Yu, Shao, Qian, George, Rockey: "Gene transfer of the neuronal NO synthase isoform to cirrhotic rat liver ameliorates portal hypertension." in: The Journal of clinical investigation, Vol. 105, Issue 6, pp. 741-8, (2000) (PubMed).

    Nathan: "Nitric oxide as a secretory product of mammalian cells." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 6, Issue 12, pp. 3051-64, (1992) (PubMed).

    Bredt, Hwang, Glatt, Lowenstein, Reed, Snyder: "Cloned and expressed nitric oxide synthase structurally resembles cytochrome P-450 reductase." in: Nature, Vol. 351, Issue 6329, pp. 714-8, (1991) (PubMed).

  • Target
    NOS1 (Nitric Oxide Synthase 1, Neuronal (NOS1))
    Alternative Name
    nNOS/NOS Type I (NOS1 Products)
    Synonyms
    NOS1 antibody, IHPS1 antibody, N-NOS antibody, NC-NOS antibody, NOS antibody, bNOS antibody, nNOS antibody, 2310005C01Rik antibody, NO antibody, NOS-I antibody, Nos-1 antibody, nos antibody, ihps1 antibody, nnos antibody, nos1 antibody, nos1-A antibody, nanos C2HC-type zinc finger 1 antibody, nitric oxide synthase 1 antibody, nitric oxide synthase 1, neuronal antibody, nitric oxide synthase 1 (neuronal) antibody, nitric oxide synthase 1 L homeolog antibody, NANOS1 antibody, NOS1 antibody, Nos1 antibody, nos1 antibody, nos1.L antibody
    Background
    Nitric oxide synthase (NOS), a cell-type specific enzyme, catalyzes the synthesis of nitric oxide (NO). NO is a short-lived radical that transmits cellular signals involved in vasorelaxation, neurotransmission, and cytotoxicity. In neurons and endothelial cells, constitutive NOS (cNOS) is activated by agonists that increase intracellular Ca2+ levels and enhance calmodulin binding. Neuronal NOS (nNOS or bNOS) and endothelial NOS (ECNOS) have recognition sites for NADPH, FAD, FMN, and calmodulin and are regulated in a similar manner. However, both have been shown to be distinct gene products of about 155 kDa and 140 kDa, respectively, and the human forms show 52% amino acid identity. Neuronal NOS and induced macrophage NOS (iNOS) share 51% amino acid homology with the greatest degree of divergence in the calmodulin binding domain. Neuronal NOS, a cytosolic protein present mainly in neural tissues, has been purified and characterized from rat cerebellum. The NO synthesized by this enzyme acts as a neurotransmitter. ECNOS has been cloned from human vascular endothelium as well as from bovine aortic endothelial cells (BAEC) and has a unique N-myristylation consensus sequence that may explain its membrane localization. This antibody is routinely tested by western blot analysis.
    Synonyms: NOS Type I, Neuronal Nitric Oxide Synthase
    Molecular Weight
    155 kDa
    Pathways
    Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction
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