SNAP25 antibody (AA 8-29)

Catalog No. ABIN967922

The Mouse Monoclonal anti-SNAP25 antibody has been validated for WB, IHC, IF and IP. It is suitable to detect SNAP25 in samples from Rat and Mouse. There are 5+ publications available.

Quick Overview for SNAP25 antibody (AA 8-29) (ABIN967922)

Target: SNAP25 (Synaptosomal-Associated Protein, 25kDa (SNAP25))

Reactivity: Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This SNAP25 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: 20-SP

Product Details anti-SNAP25 Antibody

Binding Specificity: AA 8-29

Cross-Reactivity: Rat (Rattus)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse SNAP-25 aa. 8-29

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968545, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-SNAP25 antibody (ABIN967922)

Hasegawa, Zinsser, Rhee, Vik-Mo, Davanger, Hay: "Mammalian ykt6 is a neuronal SNARE targeted to a specialized compartment by its profilin-like amino terminal domain." in: Molecular biology of the cell, Vol. 14, Issue 2, pp. 698-720, (2003) (PubMed).

Torii, Zhao, Yi, Takeuchi, Izumi: "Granuphilin modulates the exocytosis of secretory granules through interaction with syntaxin 1a." in: Molecular and cellular biology, Vol. 22, Issue 15, pp. 5518-26, (2002) (PubMed).

Martinez-Arca, Alberts, Zahraoui, Louvard, Galli: "Role of tetanus neurotoxin insensitive vesicle-associated membrane protein (TI-VAMP) in vesicular transport mediating neurite outgrowth." in: The Journal of cell biology, Vol. 149, Issue 4, pp. 889-900, (2000) (PubMed).

Chapman, An, Barton, Jahn: "SNAP-25, a t-SNARE which binds to both syntaxin and synaptobrevin via domains that may form coiled coils." in: The Journal of biological chemistry, Vol. 269, Issue 44, pp. 27427-32, (1994) (PubMed).

Oyler, Higgins, Hart, Battenberg, Billingsley, Bloom, Wilson: "The identification of a novel synaptosomal-associated protein, SNAP-25, differentially expressed by neuronal subpopulations." in: The Journal of cell biology, Vol. 109, Issue 6 Pt 1, pp. 3039-52, (1990) (PubMed).

Target Details for SNAP25

Target: SNAP25 (Synaptosomal-Associated Protein, 25kDa (SNAP25))

Alternative Name: SNAP-25

Background: Release of neurotransmitters from neurons is regulated by exocytosis of synaptic vesicles. This exocytosis is mediated by a complex consisting of membrane components of both the synaptic vesicle and the synaptic plasma membrane. The fusion complex consists of the soluble NSF (N-ethyl-maleimide-sensitive factor) and SNAPs (soluble NSF attachment proteins), along with the receptor proteins (known as SNAREs) synaptobrevin, synaptotagmin, syntaxin, and SNAP-25 (synaptosomal-associated protein of 25 kDa- the name is coincidental to the previously mentioned SNAP” terminology). SNAP-25 and syntaxin are plasmalemmal proteins (designated as t-SNAREs) while synaptobrevin and synaptotagmin are vesicular proteins (designated as v-SNAREs). These four proteins are thought to constitute an initial SNARE docking complex for regulated exocytosis. SNAP-25 lacks a transmembrane domain, but is linked to the membrane by palmitoylated cysteine residues in the central region of the molecule. This antibody is routinely tested by western blot analysis.
Synonyms: Synaptosomal Associated Protein of 25 kD

Molecular Weight: 25 kDa

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport