SMARCA2 antibody (AA 1400-1586)

Catalog No. ABIN967939

This Mouse Monoclonal antibody specifically detects SMARCA2 in WB, IHC, IF and IP. It exhibits reactivity toward Human, Mouse, Rat, Dog and Chicken. It has been mentioned in 3+ publications

Quick Overview for SMARCA2 antibody (AA 1400-1586) (ABIN967939)

Target: SMARCA2 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A, Member 2 (SMARCA2))

Reactivity: Human, Mouse, Rat, Dog, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This SMARCA2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: 24-BRM

Product Details anti-SMARCA2 Antibody

Binding Specificity: AA 1400-1586

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Dog (Canine), Chicken

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Brm aa. 1400-1586

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968535, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-SMARCA2 antibody (ABIN967939)

Wong, Shanahan, Chen, Lian, Ha, Hendricks, Ghaffari, Iliev, Penn, Woodland, Smith, Salada, Carillo, Laity, Gupte, Swedlund, Tavtigian, Teng, Lees: "BRG1, a component of the SWI-SNF complex, is mutated in multiple human tumor cell lines." in: Cancer research, Vol. 60, Issue 21, pp. 6171-7, (2000) (PubMed).

Singh, Coe, Hong: "A role for retinoblastoma protein in potentiating transcriptional activation by the glucocorticoid receptor." in: Nature, Vol. 374, Issue 6522, pp. 562-5, (1995) (PubMed).

Muchardt, Yaniv: "A human homologue of Saccharomyces cerevisiae SNF2/SWI2 and Drosophila brm genes potentiates transcriptional activation by the glucocorticoid receptor." in: The EMBO journal, Vol. 12, Issue 11, pp. 4279-90, (1993) (PubMed).

Target Details for SMARCA2

Target: SMARCA2 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A, Member 2 (SMARCA2))

Alternative Name: Brm

Background: Several of the SNF and SWI genes of Saccharomyces cerevisiae encode proteins that are involved in the regulation of transcriptional activation. One of these proteins, SNF2/SWI2, has both Drosophila and mammalian homologues, designated brm. The human Brm protein has been reported to be a 180 kDa nuclear factor that acts as a transcriptional activator when fused to a heterologus DNA binding domain. Transfected Brm, expressed in cells lacking endogenous protein, can cooperate with the glucocorticoid receptor (GR) in transcriptional activation. The cooperation between Brm and GR requires the DNA binding domain of GR and the helicase domain and the P/Q-charged domain of Brm. However, Brm does not affect on several other transcription factors. The retinoblastoma protein, Rb, stimulates the transcription of a number of genes. Like Brm, Rb up-regulates glucocorticoid-receptor-mediated transcription. Brm and Rb interact in vitro and in vivo, requiring the Rb-pocket domain and the consensus Rb-binding motif of Brm. This antibody is routinely tested by western blot analysis.

Molecular Weight: 180 kDa