Caveolin-1 antibody (AA 1-178)

Catalog No. ABIN968000

Product Details anti-Caveolin-1 Antibody

Target: Caveolin-1 (CAV1) (Caveolin 1, Caveolae Protein, 22kDa (CAV1))

Binding Specificity: AA 1-178

Reactivity: Human, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This Caveolin-1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: RSV-CEF Caveolin aa. 1-178

Clone: 2234-Caveolin 1

Isotype: IgG2a

Application Details

Comment:

Related Products: ABIN967389, ABIN968554

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-Caveolin-1 antibody (ABIN968000)

Oh, Schnitzer: "Segregation of heterotrimeric G proteins in cell surface microdomains. G(q) binds caveolin to concentrate in caveolae, whereas G(i) and G(s) target lipid rafts by default." in: Molecular biology of the cell, Vol. 12, Issue 3, pp. 685-98, (2001) (PubMed).

Razani, Zhang, Bitzer, von Gersdorff, Böttinger, Lisanti: "Caveolin-1 regulates transforming growth factor (TGF)-beta/SMAD signaling through an interaction with the TGF-beta type I receptor." in: The Journal of biological chemistry, Vol. 276, Issue 9, pp. 6727-38, (2001) (PubMed).

Schlegel, Arvan, Lisanti: "Caveolin-1 binding to endoplasmic reticulum membranes and entry into the regulated secretory pathway are regulated by serine phosphorylation. Protein sorting at the level of the endoplasmic reticulum." in: The Journal of biological chemistry, Vol. 276, Issue 6, pp. 4398-408, (2001) (PubMed).

Conrad, Smart, Ying, Anderson, Bloom: "Caveolin cycles between plasma membrane caveolae and the Golgi complex by microtubule-dependent and microtubule-independent steps." in: The Journal of cell biology, Vol. 131, Issue 6 Pt 1, pp. 1421-33, (1996) (PubMed).

Scherer, Tang, Chun, Sargiacomo, Lodish, Lisanti: "Caveolin isoforms differ in their N-terminal protein sequence and subcellular distribution. Identification and epitope mapping of an isoform-specific monoclonal antibody probe." in: The Journal of biological chemistry, Vol. 270, Issue 27, pp. 16395-401, (1995) (PubMed).

Target Details for Caveolin-1

Target: Caveolin-1 (CAV1) (Caveolin 1, Caveolae Protein, 22kDa (CAV1))

Alternative Name: Caveolin 1 (CAV1 Products)

Synonyms: fc07c04 antibody, wu:fc07c04 antibody, Cav-1 antibody, bscl3 antibody, cav1 antibody, cav1a antibody, cgl3 antibody, vip21 antibody, cav1b antibody, caveolin-1 antibody, cav antibody, mstp085 antibody, Caveolin-1 antibody, CAV1 antibody, cav-1 antibody, Cav antibody, caveolin 1 antibody, BSCL3 antibody, CGL3 antibody, MSTP085 antibody, PPH3 antibody, VIP21 antibody, caveolin 1 antibody, caveolin 1 L homeolog antibody, caveolin 1 S homeolog antibody, caveolin 1, caveolae protein antibody, caveolin 1, caveolae protein, 22kDa antibody, Caveolin antibody, cav1 antibody, cav1.L antibody, cav1.S antibody, CAV1 antibody, Cav1 antibody, cav-1 antibody

Background: Identified as a tyrosine phosphorylated protein in Rous sarcoma virus-transformed chick embryo fibroblasts (CEF), caveolin is now known to be ubiquitously expressed. Caveolin (also known as VIP21) localizes to non-clathrin membrane invaginations (caveolae) on the inner surface of the plasma membrane. This transmembrane protein plays a structural role in these specializations. Caveolin is also present at the trans-Golgi network (TGN) and similar quantities are found in apically and basolaterally destined transport vesicles. Caveolin is part of a complex containing glycosylphosphatidylinositol (GPI)-linked molecules and cytoplasmic signaling proteins. Caveolin is a transmembrane adaptor molecule that can simultaneously recognize GPI-linked proteins and interact with downstream cytoplasmic signaling molecules, such as c-yes, Annexin II, and hetero-trimeric G proteins. Caveolin-1 can generate two forms, alpha and beta, due to alternate splicing of the mRNA. Caveolin-1 forms large lipid-binding homo-oligomers which are believed to play a role in caveolae formation. It may also function as a scaffolding protein which concentrates and organizes signaling molecules, a role supported by the fact that caveolin-1 interacts directly with inactive Ras and G-protein alpha subunits. This antibody is routinely tested by western blot analysis.

Molecular Weight: 22 kDa

Pathways: Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of Transporter Activity, VEGFR1 Specific Signals