HCLS1 antibody (AA 17-190)

Catalog No. ABIN968027

Product Details anti-HCLS1 Antibody

Target: HCLS1 (Hematopoietic Cell-Specific Lyn Substrate 1 (HCLS1))

Binding Specificity: AA 17-190

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HCLS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human HS1 aa. 17-190

Clone: 9-HS1

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968536, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-HCLS1 antibody (ABIN968027)

Fukuda, Kitamura, Taniuchi, Maekawa, Benhamou, Sarthou, Watanabe: "Restoration of surface IgM-mediated apoptosis in an anti-IgM-resistant variant of WEHI-231 lymphoma cells by HS1, a protein-tyrosine kinase substrate." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 16, pp. 7302-6, (1995) (PubMed).

Takemoto, Furuta, Li, Strong-Sparks, Hashimoto: "LckBP1, a proline-rich protein expressed in haematopoietic lineage cells, directly associates with the SH3 domain of protein tyrosine kinase p56lck." in: The EMBO journal, Vol. 14, Issue 14, pp. 3403-14, (1995) (PubMed).

Yamanashi, Okada, Semba, Yamori, Umemori, Tsunasawa, Toyoshima, Kitamura, Watanabe, Yamamoto: "Identification of HS1 protein as a major substrate of protein-tyrosine kinase(s) upon B-cell antigen receptor-mediated signaling." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 8, pp. 3631-5, (1993) (PubMed).

Target Details for HCLS1

Target: HCLS1 (Hematopoietic Cell-Specific Lyn Substrate 1 (HCLS1))

Alternative Name: HS1 (HCLS1 Products)

Synonyms: CTTNL antibody, HS1 antibody, AW213261 antibody, HCLS1 antibody, hcls1 antibody, si:dkey-9a20.6 antibody, wu:fk96b08 antibody, zgc:154014 antibody, hematopoietic cell-specific Lyn substrate 1 antibody, hematopoietic cell specific Lyn substrate 1 antibody, hematopoietic lineage cell-specific protein-like antibody, HCLS1 antibody, Hcls1 antibody, LOC100223649 antibody, hcls1 antibody

Background: HS1 (lck-binding protein 1 (lckBP1)) is a protein of 486 amino acids with homology to cytoplasmic tyrosine kinases. The protein contains an SH3 domain and two proline-rich regions, that may be the binding site for lck. Co- mmunoprecipitation experiments demonstrated a tight association between HS1 and lck. The SH3 domain of lck is necessary for this interaction. In addition, HS1 contains tandem repeat sequence of seven amino acids which is a substrate for the tyrosine kinase src. This sequence is also found in the protein cortactin. Therefore, HS1, like cortactin, may be an important adaptor that is critical for the regulation of cellular tyrosine kinases. This antibody is routinely tested by western blot analysis.

Molecular Weight: 75 kDa

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Myometrial Relaxation and Contraction, Maintenance of Protein Location