RAC1 antibody

Catalog No. ABIN968088

The Mouse Monoclonal anti-RAC1 antibody (Clone 102-Rac1) (ABIN968088) specifically detects RAC1 in WB, IHC, IF and IP. The antibody is reactive with Human, Mouse, Rat, Dog and Chicken samples.

Quick Overview for RAC1 antibody (ABIN968088)

Target: RAC1

Reactivity: Human, Mouse, Rat, Dog, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This RAC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: 102-Rac1

Product Details anti-RAC1 Antibody

Cross-Reactivity: Chicken, Dog (Canine), Mouse (Murine), Rat (Rattus)

Characteristics: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Positive Control: Rat Cerebrum

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Isotype: IgG2b kappa

Application Details

Application Notes: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Aqueous buffered solution containing BSA, glycerol.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

Target Details for RAC1

Target: RAC1

Background: Rac1 is a member of the expanding family of Ras-related GTPase proteins. These small proteins have the ability to switch between two different conformational states: the active (GTP-bound) and the inactive (GDP-bound). The GTPase activity is increased by specific activator proteins named GAPs. Rac1, contains the consensus sequence Cys-X-X-X-COOH, which localizes it to the plasma membrane. Growth factor stimulation of cells results in a dramatic reorganization of the actin filament network and membrane ruffling, processes for which Rac1 is essential. Constitutive expression of Rac1 leads to cell transformation. This process is mediated by the Rac1-dependent activation of the p65PAK serine/threonine kinase. IQGAP1 has been identified as a GTPase activating protein specific for Rac1.
Testing in native ELISA with recombinant proteins to CDC42 and Rho have shown that the antibody to Rac1 (cat No ABIN968088/1) recognizes CDC42 and Rho. Due to sequence homology among various small GTPase familyl members, potential reactivity could be observed with other family members.

Molecular Weight: 21 kDa

Pathways: WNT Signaling, Regulation of Actin Filament Polymerization, Cell-Cell Junction Organization, Thromboxane A2 Receptor Signaling, VEGF Signaling