Sec8 antibody (AA 31-201)

Catalog No. ABIN968092

Product Details anti-Sec8 Antibody

Target: Sec8 (EXOC4) (Exocyst Complex Component 4 (EXOC4))

Binding Specificity: AA 31-201

Reactivity: Human, Mouse, Rat, Dog, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This Sec8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Cross-Reactivity: Dog (Canine), Mouse (Murine), Chicken, Human

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat Sec8 aa. 31-201

Clone: 14-Sec8

Isotype: IgG2b

Application Details

Comment:

Related Products: ABIN968619, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-Sec8 antibody (ABIN968092)

Charron, Nakamura, Bacallao, Wandinger-Ness: "Compromised cytoarchitecture and polarized trafficking in autosomal dominant polycystic kidney disease cells." in: The Journal of cell biology, Vol. 149, Issue 1, pp. 111-24, (2000) (PubMed).

Duncan, Don-Wauchope, Tapechum, Shipston, Chow, Estibeiro: "High-efficiency Semliki Forest virus-mediated transduction in bovine adrenal chromaffin cells." in: The Biochemical journal, Vol. 342 Pt 3, pp. 497-501, (1999) (PubMed).

Ting, Hazuka, Hsu, Kirk, Bean, Scheller: "rSec6 and rSec8, mammalian homologs of yeast proteins essential for secretion." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 21, pp. 9613-7, (1995) (PubMed).

Target Details for Sec8

Target: Sec8 (EXOC4) (Exocyst Complex Component 4 (EXOC4))

Alternative Name: Sec8 (EXOC4 Products)

Synonyms: CG2095 antibody, Dmel\\CG2095 antibody, Sec8 antibody, Sec8p antibody, dsec8 antibody, fun antibody, SEC8L1 antibody, DKFZp459N1430 antibody, SEC8 antibody, C78892 antibody, Sec8l1 antibody, ALR antibody, si:ch211-92l17.2 antibody, si:rp71-18a24.2 antibody, ATSEC8 antibody, SUBUNIT OF EXOCYST COMPLEX 8 antibody, subunit of exocyst complex 8 antibody, Secretory 8 antibody, exocyst complex component 4 antibody, exocyst complex component 4 L homeolog antibody, Exocyst complex component 4 antibody, subunit of exocyst complex 8 antibody, Sec8 antibody, EXOC4 antibody, CpipJ_CPIJ001354 antibody, Exoc4 antibody, exoc4 antibody, exoc4.L antibody, sec-8 antibody, SEC8 antibody

Background: Signal transmission between neurons is regulated by the release of neurotransmitters at the synapse. This process, which is controlled by a complex pathway of membrane trafficking in the presynaptic nerve terminal, leads to membrane fusion and neurotransmitter secretion. Sec8 is a hydrophilic protein of 975 amino acids that is highly expressed in brain and kidney. It is homologus with the yeast secretory protein, Sec8p. In yeast, Sec8 is essential for the Golgi-to-plasma membrane traffic of proteins during constitutive secretion. In rat brain, Sec8 colocalizes with Rab3 and Syntaxin-1a, two important proteins that regulate the fusion of the synaptic vesicle to the plasma membrane. The primary structure of Sec8, a coiled-coil domain (residues 34-99), is commonly found in other proteins important in secretory pathways. This antibody is routinely tested by western blot analysis.

Molecular Weight: 110 kDa

Pathways: Peptide Hormone Metabolism, Synaptic Vesicle Exocytosis