ENAH antibody (AA 415-541)

Catalog No. ABIN968115

This anti-ENAH antibody is a Mouse Monoclonal antibody detecting ENAH in WB, IHC, IP and IF. Suitable for Human, Mouse, Rat and Chicken. This Primary Antibody has been cited in 4+ publications.

Quick Overview for ENAH antibody (AA 415-541) (ABIN968115)

Target: ENAH (Enabled Homolog (ENAH))

Reactivity: Human, Mouse, Rat, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This ENAH antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Clone: 21-Mena

Product Details anti-ENAH Antibody

Binding Specificity: AA 415-541

Cross-Reactivity: Human, Rat (Rattus), Chicken

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse Mena aa. 415-541

Isotype: IgA

Application Details

Comment:

Related Products: ABIN968552, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-ENAH antibody (ABIN968115)

Howe, Hogan, Juliano: "Regulation of vasodilator-stimulated phosphoprotein phosphorylation and interaction with Abl by protein kinase A and cell adhesion." in: The Journal of biological chemistry, Vol. 277, Issue 41, pp. 38121-6, (2002) (PubMed).

García Arguinzonis, Galler, Walter, Reinhard, Simm: "Increased spreading, Rac/p21-activated kinase (PAK) activity, and compromised cell motility in cells deficient in vasodilator-stimulated phosphoprotein (VASP)." in: The Journal of biological chemistry, Vol. 277, Issue 47, pp. 45604-10, (2002) (PubMed).

Drees, Friederich, Fradelizi, Louvard, Beckerle, Golsteyn: "Characterization of the interaction between zyxin and members of the Ena/vasodilator-stimulated phosphoprotein family of proteins." in: The Journal of biological chemistry, Vol. 275, Issue 29, pp. 22503-11, (2000) (PubMed).

Gertler, Niebuhr, Reinhard, Wehland, Soriano: "Mena, a relative of VASP and Drosophila Enabled, is implicated in the control of microfilament dynamics." in: Cell, Vol. 87, Issue 2, pp. 227-39, (1996) (PubMed).

Target Details for ENAH

Target: ENAH (Enabled Homolog (ENAH))

Alternative Name: Mena

Background: Abl and Disabled (Dab) are essential for correct axonal connections during Drosophila development. Mutations in Enabled (Ena) rescue the genetic defects caused by mutated Abl and Dab. Mena (Mammalian Ena) is a protein with significant homology to Eva, VASP, and WASP proteins. The murine Mena gene predicts a protein of 541 amino acids. However, two additional exons that could introduce 246 amino acids onto the C-terminal region have been identified. Homology searches identified two EVH domains (Ena-Vasp homology), one near each terminus of Mena. Antibodies to Mena detect 80 kDa, 88 kDa, and 140 kDa protein with the 80 kDa and 88 kDa proteins being widely expressed and the 140 kDa protein reportedly mainly found in brain tissue. Like VASP, Mena is localized to focal contacts and, when ectopically expressed, induces the formation of F-actin outgrowths in fibroblasts. This antibody is routinely tested by Western blot analysis.
Synonyms: Mammalian Ena (Enabled)

Molecular Weight: 140, 88, 80 kDa