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AKAP1 antibody (AA 66-212)

AKAP1 Reactivity: Human, Rat WB, IHC, IF, IP Host: Mouse Monoclonal 6-AKAP149 unconjugated
Catalog No. ABIN968136
  • Target See all AKAP1 Antibodies
    AKAP1 (A Kinase (PRKA) Anchor Protein 1 (AKAP1))
    Binding Specificity
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 66-212
    Reactivity
    • 28
    • 2
    • 1
    Human, Rat
    Host
    • 25
    • 3
    Mouse
    Clonality
    • 28
    Monoclonal
    Conjugate
    • 21
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This AKAP1 antibody is un-conjugated
    Application
    • 18
    • 11
    • 10
    • 8
    • 7
    • 5
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
    Cross-Reactivity
    Rat (Rattus)
    Characteristics
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Human AKAP149 aa. 66-212
    Clone
    6-AKAP149
    Isotype
    IgG1
    Top Product
    Discover our top product AKAP1 Primary Antibody
  • Comment

    Related Products: ABIN968535, ABIN967389

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    250 μg/mL
    Buffer
    Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store undiluted at -20° C.
  • Schillace, Andrews, Liberty, Davey, Carr: "Identification and characterization of myeloid translocation gene 16b as a novel a kinase anchoring protein in T lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 168, Issue 4, pp. 1590-9, (2002) (PubMed).

    Tanji, Yamamoto, Yorioka, Kohno, Kikuchi, Kikuchi: "A-kinase anchoring protein AKAP220 binds to glycogen synthase kinase-3beta (GSK-3beta ) and mediates protein kinase A-dependent inhibition of GSK-3beta." in: The Journal of biological chemistry, Vol. 277, Issue 40, pp. 36955-61, (2002) (PubMed).

    Steen, Collas: "Mistargeting of B-type lamins at the end of mitosis: implications on cell survival and regulation of lamins A/C expression." in: The Journal of cell biology, Vol. 153, Issue 3, pp. 621-6, (2001) (PubMed).

    DellAcqua, Scott: "Protein kinase A anchoring." in: The Journal of biological chemistry, Vol. 272, Issue 20, pp. 12881-4, (1997) (PubMed).

    Trendelenburg, Hummel, Riecken, Hanski: "Molecular characterization of AKAP149, a novel A kinase anchor protein with a KH domain." in: Biochemical and biophysical research communications, Vol. 225, Issue 1, pp. 313-9, (1996) (PubMed).

  • Target
    AKAP1 (A Kinase (PRKA) Anchor Protein 1 (AKAP1))
    Alternative Name
    AKAP149 (AKAP1 Products)
    Synonyms
    AKAP1 antibody, akap1 antibody, fa08b04 antibody, wu:fa08b04 antibody, AKAP antibody, AKAP121 antibody, AKAP149 antibody, AKAP84 antibody, D-AKAP1 antibody, PPP1R43 antibody, PRKA1 antibody, SAKAP84 antibody, TDRD17 antibody, Akap antibody, C76494 antibody, C81186 antibody, DAKAP1 antibody, S-AKAP84 antibody, Akap84 antibody, A-kinase anchoring protein 1 antibody, A kinase (PRKA) anchor protein 1b antibody, A kinase (PRKA) anchor protein 1 antibody, AKAP1 antibody, akap1b antibody, akap1 antibody, Akap1 antibody
    Background
    The targeting of protein kinases and phosphatases to cellular compartments in a timely manner may provide specificity in signal transduction events. For instance, attachment of type II cAMP-dependent Protein Kinase (PKA) to the cytoskeleton occurs through the binding of the regulatory subunit (RII) to microtubule associated-protein 2. Several proteins, such as microtubule-associated protein 2, have been identified as PKA type I/II anchoring proteins and form a family named AKAP (A-Kinase Anchor Proteins). AKAP149 is a protein of 903 amino acids. It has a PKA [RII] binding site at amino acids 355-376 preceded by a leucine zipper domain. AKAP149 mRNA has been reported to be expressed ubiquitously, with the highest levels in prostate and small intestine. AKAP149 contains a KH domain characteristic of RNA-binding proteins. Thus, AKAP149 is a PKA [RII] anchoring protein that may target this kinase to sites of RNA processing for regulation of RNA binding protein activity. This antibody is routinely tested by western blot analysis.
    Molecular Weight
    149 kDa
    Pathways
    Synaptic Membrane
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