MAP2K5 antibody (AA 13-188)

Catalog No. ABIN968272

This Mouse Monoclonal antibody specifically detects MAP2K5 in WB and IF. It exhibits reactivity toward Human and has been mentioned in 2+ publications.

Quick Overview for MAP2K5 antibody (AA 13-188) (ABIN968272)

Target: MAP2K5 (Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MAP2K5 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: LPR-01

Product Details anti-MAP2K5 Antibody

Binding Specificity: AA 13-188

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Dog (Canine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human MEK5

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968537, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 µg/ml

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-MAP2K5 antibody (ABIN968272)

English, Vanderbilt, Xu, Marcus, Cobb: "Isolation of MEK5 and differential expression of alternatively spliced forms." in: The Journal of biological chemistry, Vol. 270, Issue 48, pp. 28897-902, (1996) (PubMed).

Zhou, Bao, Dixon: "Components of a new human protein kinase signal transduction pathway." in: The Journal of biological chemistry, Vol. 270, Issue 21, pp. 12665-9, (1995) (PubMed).

Target Details for MAP2K5

Target: MAP2K5 (Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5))

Alternative Name: MEK5

Background: The mitogen-activated protein (MAP) kinase cascade participates in the Ras signal transduction pathway. The MAP kinase cascade consists of MEK kinase (Raf-1 or B-Raf), MAP/ERK kinase (MEK1 or MEK2), and extracellular-regulated protein kinase (ERK1 or ERK2). Raf becomes activated following its interaction with Ras-GTP (activated Ras). Via phosphorylation reactions, Raf activates MEK which, in turn, activates ERK. MEK5, also known as MAP Kinase 5, is a 50 kDa protein that is 40% identical to MEK1 and MEK2. Alternative splicing results in two MEK5 isoforms: alpha (50 kDa) and beta (40 kDa). MEK5beta has been reported to be ubiquitously expressed while MEK5alpha is expressed primarily in the liver and brain. The N-terminal amino acid sequence of MEK5alpha resembles sequences found in actin cytoskeletal proteins, indicating that MEK5alpha may associate with the cytoskeleton. However, the role of MEK5 in the MAP kinase pathway and its substrate remain to be identified. This antibody is routinely tested by western blot analysis.
Synonyms: MAP Kinase-5

Molecular Weight: 50 kDa

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway