PIK3CD antibody (AA 73-90)

Catalog No. ABIN968316

This anti-PIK3CD antibody is a Mouse Monoclonal antibody detecting PIK3CD in WB and IF. Suitable for Mouse and Rat. This Primary Antibody has been cited in 1 publication.

Quick Overview for PIK3CD antibody (AA 73-90) (ABIN968316)

Target: PIK3CD (Phosphoinositide-3-Kinase, Catalytic, delta Polypeptide (PIK3CD))

Reactivity: Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This PIK3CD antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 29-PI3 Kinase p110delta

Product Details anti-PIK3CD Antibody

Binding Specificity: AA 73-90

Cross-Reactivity: Rat (Rattus)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse PI3 Kinase p110delta aa. 73-90

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968545, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-PIK3CD antibody (ABIN968316)

Chantry, Vojtek, Kashishian, Holtzman, Wood, Gray, Cooper, Hoekstra: "p110delta, a novel phosphatidylinositol 3-kinase catalytic subunit that associates with p85 and is expressed predominantly in leukocytes." in: The Journal of biological chemistry, Vol. 272, Issue 31, pp. 19236-41, (1997) (PubMed).

Target Details for PIK3CD

Target: PIK3CD (Phosphoinositide-3-Kinase, Catalytic, delta Polypeptide (PIK3CD))

Alternative Name: PI3-Kinase p110 delta

Background: Phosphoinositide 3 Kinase (PI3 Kinase) phosphorylates the D-3 position of the inositol ring of phosphatidylinositol, producing PtdIns(3)P, PtdIns(3,4)P2, and PtdIns (3,4,5)P3. PI3-kinase is a heterodimer of an 85 kDa regulatory subunit (p85) containing two SH2 domains and an SH3 domain, and a 110 kDa catalytic subunit (p110). Four isoforms of p110 have been identified (alpha , beta , gamma, and delta). The p110alpha, beta isoforms are distributed ubiquitously. The p110delta isoform is found exclusively in lymphocytes and lymphoid tissues. Lymphocytes also contains p110alpha and both isoforms interact with p85 and are recruited to activated signaling complexes. However, several biochemical and structural differences distinguish p110delta. Unlike p110alpha, p110delta autophosphorylates and does not phosphorylate p85. In addition, p110delta contains a Pro-rich region, which endows it with the potential for interaction with SH3 domain-containing proteins, and a bZIP-like domain, which is a common transcription factor domain that has also been identified in certain protein kinases. With the established role of PI3-Ks in cytoskeletal rearrangements, it is thought that p110delta contributes specifically to the regulation of lymphocytes extravasation into tissues. There is a high degree of homology in the immunogen region with the P110beta form (77%), and it is likely that our antibody will detect both the beta and delta forms.

Molecular Weight: 110 kDa

Pathways: BCR Signaling, Warburg Effect