CHEK2 antibody (AA 31-234)

Catalog No. ABIN968596

This Mouse Monoclonal antibody specifically detects CHEK2 in WB and IF. It exhibits reactivity toward Human, Mouse and Ratand has been mentioned in 1 publication.

Quick Overview for CHEK2 antibody (AA 31-234) (ABIN968596)

Target: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CHEK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 19-Chk2

Product Details anti-CHEK2 Antibody

Binding Specificity: AA 31-234

Cross-Reactivity: Human, Rat (Rattus)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse Chk2 aa. 31-234

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-CHEK2 antibody (ABIN968596)

Tominaga, Morisaki, Kaneko, Fujimoto, Tanaka, Ohtsubo, Hirai, Okayama, Ikeda, Nakanishi: "Role of human Cds1 (Chk2) kinase in DNA damage checkpoint and its regulation by p53." in: The Journal of biological chemistry, Vol. 274, Issue 44, pp. 31463-7, (1999) (PubMed).

Target Details for CHEK2

Target: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Alternative Name: Chk2

Background: The cell cycle is regulated by multiple checkpoints that determine cell fate. Such checkpoints ensure that DNA replication and chromosomal segregation are completed with high fidelity. When DNA is damaged, specific kinases and phosphatases, key components of cell cycle checkpoints, function to arrest the cell cycle and provide the necessary time for DNA repair. For example, DNA damage induces arrest of the cell cycle at the G2 checkpoint via Wee1-mediated inhibitory phosphorylation of the kinase Cdc2. Inhibition is relieved by the Cdc25C phosphatase via Cdc2 dephosphorylation. In turn, the Chk1 and Chk2 protein kinases phosphorylate and inhibit Cdc25C, thus preventing activation of the Cdc2-cyclin B complex and entry into mitosis. Chk2, the human homolog of S. cerevisiae Rad53 and S. pombe Cds1, contains a C-terminal kinase domain, an N-terminal regulatory region that is rich in TQ and SQ pairs, and a forked head-associated domain (FHA) which is found in other cell cycle kinases. Chk2 is expressed during late G1 to M phase and is found in the nucleoplasm. Thus, Chk1 and Chk2 activity may be an important checkpoint that inhibits entry into mitosis in response to DNA damage. This antibody is routinely tested by western blot analysis.

Molecular Weight: 60 kDa

Pathways: p53 Signaling, Apoptosis, Cell Division Cycle