KSR1 antibody (AA 90-203)

Catalog No. ABIN968599

This Mouse Monoclonal antibody specifically detects KSR1 in WB and IF. It exhibits reactivity toward Mouse and Rat and has been mentioned in 4+ publications.

Quick Overview for KSR1 antibody (AA 90-203) (ABIN968599)

Target: KSR1 (Kinase Suppressor of Ras 1 (KSR1))

Reactivity: Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This KSR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 15-KSR

Product Details anti-KSR1 Antibody

Binding Specificity: AA 90-203

Cross-Reactivity: Rat (Rattus)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse KSR-1 aa. 90-203

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-KSR1 antibody (ABIN968599)

Stewart, Sundaram, Zhang, Lee, Han, Guan: "Kinase suppressor of Ras forms a multiprotein signaling complex and modulates MEK localization." in: Molecular and cellular biology, Vol. 19, Issue 8, pp. 5523-34, (1999) (PubMed).

Michaud, Therrien, Cacace, Edsall, Spiegel, Rubin, Morrison: "KSR stimulates Raf-1 activity in a kinase-independent manner." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 24, pp. 12792-6, (1998) (PubMed).

Zhang, Yao, Delikat, Bayoumy, Lin, Basu, McGinley, Chan-Hui, Lichenstein, Kolesnick: "Kinase suppressor of Ras is ceramide-activated protein kinase." in: Cell, Vol. 89, Issue 1, pp. 63-72, (1997) (PubMed).

Therrien, Chang, Solomon, Karim, Wassarman, Rubin: "KSR, a novel protein kinase required for RAS signal transduction." in: Cell, Vol. 83, Issue 6, pp. 879-88, (1996) (PubMed).

Target Details for KSR1

Target: KSR1 (Kinase Suppressor of Ras 1 (KSR1))

Alternative Name: KSR-1

Background: Proteins of the Ras superfamily play critical roles in the control of normal and neoplastic proliferation. In mammalian cells there are four true Ras proteins (encoded by Ha-ras, N-ras, Ki-rasA, and Ki-rasB). These proteins relay signals from tyrosine kinases at the plasma membrane to the nucleus via a network of Ser/Thr kinases that includes the MAP kinase (Raf-MEK-ERK) pathway. Kinase suppressor of Ras (KSR-1) was discovered in a genetic screen to identify mutations that suppress constitutively active Ras mutants. KSR-1 contains five domains that are conserved areas (CA) in Drosophila, mouse, and human. CA1 is a unique domain, CA2 is a proline-rich domain, CA3 is a cysteine-rich domain, CA4 is a Ser/Thr rich region, and CA5 contains a C-terminal kinase domain similar to that of Raf. In COS7 cells, ceramide induces the autophosphorylation of KSR-1 and KSR-1-induced phosphorylation and activation of Raf. In addition, overexpression of KSR-1 in 293T cells leads to recruitment of MEK to a 700 kDa protein complex and translocates MEK from the soluble to the membrane-associated fraction. Thus, KSR-1 may have important kinase and scaffolding activities during Ras-related signaling. This antibody is routinely tested by western blot analysis.

Molecular Weight: 115 kDa