Nestin antibody (AA 402-604)

Catalog No. ABIN968628

Product Details anti-Nestin Antibody

Target: Nestin (NES)

Binding Specificity: AA 402-604

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nestin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Cross-Reactivity: Human

Characteristics: 1. Please refer to us for technical protocols.
2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat Nestin aa. 402-604 Recombinant Protein

Clone: 25-NESTIN

Isotype: IgG1 kappa

Application Details

Comment:

Related Products: ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-Nestin antibody (ABIN968628)

Jin, Zhu, Sun, Mao, Xie, Greenberg: "Vascular endothelial growth factor (VEGF) stimulates neurogenesis in vitro and in vivo." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 18, pp. 11946-50, (2002) (PubMed).

Kernie, Erwin, Parada: "Brain remodeling due to neuronal and astrocytic proliferation after controlled cortical injury in mice." in: Journal of neuroscience research, Vol. 66, Issue 3, pp. 317-26, (2001) (PubMed).

Steinert, Chou, Prahlad, Parry, Marekov, Wu, Jang, Goldman: "A high molecular weight intermediate filament-associated protein in BHK-21 cells is nestin, a type VI intermediate filament protein. Limited co-assembly in vitro to form heteropolymers with type III vimentin and type IV alpha-internexin." in: The Journal of biological chemistry, Vol. 274, Issue 14, pp. 9881-90, (1999) (PubMed).

Kachinsky, Dominov, Miller: "Myogenesis and the intermediate filament protein, nestin." in: Developmental biology, Vol. 165, Issue 1, pp. 216-28, (1994) (PubMed).

Lendahl, Zimmerman, McKay: "CNS stem cells express a new class of intermediate filament protein." in: Cell, Vol. 60, Issue 4, pp. 585-95, (1990) (PubMed).

Target Details for Nestin

Target: Nestin (NES)

Alternative Name: Nestin (NES Products)

Synonyms: paranemin antibody, NES antibody, AA166324 antibody, C78523 antibody, ESTM46 antibody, nestin antibody, nestin S homeolog antibody, NES antibody, nes.S antibody, Nes antibody, nes antibody

Background: The cytoskeleton consists primarily of core structural proteins that include microfilaments, microtubules, and intermediate filaments (IFs). IFs contain more than 50 distinct proteins that are organized into six different subtypes: Type I/II keratins expressed in epithelia, type III vimentin/desmin, type IV neurofilament proteins, type V nuclear lamins, and type VI nestin expressed primarily in embryonic cells. Nestin has a conserved core region (amino acids 7 to 314), which contains an alpha helical domain that is involved in coiled-coil assembly of IFs. The C-terminal region of nestin is similar to type IV IFs, since it contains highly charged amino acids, many glutamate residues, and an 11 amino acid repeat motif. Nestin is expressed in the cerebrum during embryonic development, in the cerebellum during early postnatal development, and in dermatomal cells and myoblasts during myogenesis. In vitro, nestin forms homodimers and homotetramers, but not IFs, and can co-assemble with type III vimentin and type IV internexin proteins. Thus, nestin is a core IF protein that is essential for proper cytoskeletal formation during neurogenesis and myogenesis.

Molecular Weight: 200-260 kDa