TOPBP1 antibody (AA 204-416)

Catalog No. ABIN968690

The Mouse Monoclonal anti-TOPBP1 antibody has been validated for WB and IF. It is suitable to detect TOPBP1 in samples from Human. There are 3+ publications available.

Quick Overview for TOPBP1 antibody (AA 204-416) (ABIN968690)

Target: TOPBP1 (Topoisomerase (DNA) II Binding Protein 1 (TOPBP1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TOPBP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 33-TopBP1

Product Details anti-TOPBP1 Antibody

Binding Specificity: AA 204-416

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human TopBP1 aa. 204-416

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968537, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-TOPBP1 antibody (ABIN968690)

Yamane, Katayama, Tsuruo: "The BRCT regions of tumor suppressor BRCA1 and of XRCC1 show DNA end binding activity with a multimerizing feature." in: Biochemical and biophysical research communications, Vol. 279, Issue 2, pp. 678-84, (2001) (PubMed).

Yamane, Tsuruo: "Conserved BRCT regions of TopBP1 and of the tumor suppressor BRCA1 bind strand breaks and termini of DNA." in: Oncogene, Vol. 18, Issue 37, pp. 5194-203, (1999) (PubMed).

Yamane, Kawabata, Tsuruo: "A DNA-topoisomerase-II-binding protein with eight repeating regions similar to DNA-repair enzymes and to a cell-cycle regulator." in: European journal of biochemistry / FEBS, Vol. 250, Issue 3, pp. 794-9, (1998) (PubMed).

Target Details for TOPBP1

Target: TOPBP1 (Topoisomerase (DNA) II Binding Protein 1 (TOPBP1))

Alternative Name: TopBP1

Background: Eukaryotic DNA topoisomerase II, a ubiquitous ATP-dependent type II topoisomerase, is an essential nuclear enzyme in DNA replication and transcription, chromatin segregation, and cell cycle progression. Topoisomerases transiently break a pair of complementary strands in double-stranded DNA to form a gate for the passage of duplex DNA. Two isoforms of DNA topoisomerase II have been identified: topo IIalpha and topo IIbeta. These exhibit a high degree of homology, except for some divergence in the C-terminal region. Both contain multiple bipartite nuclear localization sequences (NLS) that mediate their subnuclear localization. DNA Topoisomerase II Binding Protein 1 (TopBP1) binds the C-terminal region of Topo IIbeta via its N-terminal region. In addition, TopBP1 contains a putative ADP-ribosylation site, two N-terminal NLS domains, and 8 repeating BRCA1 C-terminal (BRCT) domains found in DNA repair proteins, such as BRCA1, XRCC1, and Rad4. The BRCT domains of TopBP1 have been shown to bind DNA breaks, DNA nicks, and DNA termini, but not circular intact DNA. Thus, TopBP1 may localize topoisomerases to sites of DNA damage.
Synonyms: Topoisomerase II Binding Protein 1

Molecular Weight: 161 kDa

Pathways: Chromatin Binding