SMARCB1 antibody (AA 257-359)

Catalog No. ABIN968742

Cited in 3+ publications. The Mouse Monoclonal anti-SMARCB1 antibody (Clone 25-BAF47) (ABIN968742) specifically detects SMARCB1 in WB and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for SMARCB1 antibody (AA 257-359) (ABIN968742)

Target: SMARCB1 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily B, Member 1 (SMARCB1))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This SMARCB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 25-BAF47

Product Details anti-SMARCB1 Antibody

Binding Specificity: AA 257-359

Cross-Reactivity: Rat (Rattus), Human

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse BAF47 aa. 257-359

Isotype: IgG2a

Application Details

Comment:

Related Products: ABIN968540, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-SMARCB1 antibody (ABIN968742)

Guidi, Sands, Zambrowicz, Turner, Demers, Webster, Smith, Imbalzano, Jones: "Disruption of Ini1 leads to peri-implantation lethality and tumorigenesis in mice." in: Molecular and cellular biology, Vol. 21, Issue 10, pp. 3598-603, (2001) (PubMed).

Bruder, Dumanski, Kedra: "The mouse ortholog of the human SMARCB1 gene encodes two splice forms." in: Biochemical and biophysical research communications, Vol. 257, Issue 3, pp. 886-90, (1999) (PubMed).

Kalpana, Marmon, Wang, Crabtree, Goff: "Binding and stimulation of HIV-1 integrase by a human homolog of yeast transcription factor SNF5." in: Science (New York, N.Y.), Vol. 266, Issue 5193, pp. 2002-6, (1995) (PubMed).

Target Details for SMARCB1

Target: SMARCB1 (SWI/SNF Related, Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily B, Member 1 (SMARCB1))

Alternative Name: BAF47

Background: SWI/SNF complexes facilitate gene activation and transcription factor binding by altering repressive chromatin structures in an ATP-dependent manner. In mammals, SWI/SNF complexes are present in mutliple forms that include 9-12 BRG1-associated factors (BAFs) per complex. These BAF proteins range in molecular weight from 47 to 250 kDa. BAF47/SNF5 (SMARCB1/Ini1) complexes with BRG1- and BRM-containing SWI/SNF complexes. BAF47/SNF5 mRNA is widely expressed in cell lines and tissues, and BAF47/SNF5 gene is mutated in many human tumors. The latter is indicative of a tumor suppressor role for BAF47/SNF5. In addition, mice deficient for BAF47 die early in embryogenesis, while BAF47/SNF5 heterozygous mice display a variety of tumors in the soft tissues of the head and neck. BAF47/SNF5 also binds the HIV-1 integrase and stimulates integrase-mediated DNA joining activity. Thus, BAF47/SNF5 is a component of SWI/SNF complexes that may be critical for normal development and tumor suppression, but may also be a protein utilized for viral DNA integration into host DNA. This antibody is routinely tested by western blot analysis.
Synonyms: SNF5

Molecular Weight: 47 kDa