S100B antibody (AA 1-92)

Catalog No. ABIN968841

This anti-S100B antibody is a Mouse Monoclonal antibody detecting S100B in WB, IF and BI. Suitable for Human, Rat and Mouse. This Primary Antibody has been cited in 2+ publications.

Quick Overview for S100B antibody (AA 1-92) (ABIN968841)

Target: S100B (S100 Calcium Binding Protein B (S100B))

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This S100B antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), BioImaging (BI)

Clone: 19-S100B

Product Details anti-S100B Antibody

Binding Specificity: AA 1-92

Cross-Reactivity: Rat (Rattus), Human

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse S100B aa. 1-92

Isotype: IgG1

Application Details

Application Notes: Bioimaging:
Methanol Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 90% methanol. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Wash three times with PBS. Flick out PBS and add second step reagent. Incubate for 1 hour at RT. Wash three times with PBS.
Triton-X 100 Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 0.1% Triton-X 100. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Flick out and wash three times with PBS. Flick out and add second step reagent. Incubate for 1 hour at RT. Flick out and wash three times with PBS.

Comment:

Related Products: ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-S100B antibody (ABIN968841)

Scotto, Delphin, Deloulme, Baudier: "Concerted regulation of wild-type p53 nuclear accumulation and activation by S100B and calcium-dependent protein kinase C." in: Molecular and cellular biology, Vol. 19, Issue 10, pp. 7168-80, (2000) (PubMed).

Jiang, Shah, Hilt: "Organization, sequence, and expression of the murine S100 beta gene. Transcriptional regulation by cell type-specific cis-acting regulatory elements." in: The Journal of biological chemistry, Vol. 268, Issue 27, pp. 20502-11, (1993) (PubMed).

Target Details for S100B

Target: S100B (S100 Calcium Binding Protein B (S100B))

Alternative Name: S100B MAB

Background: The S100 multigene family consists of a number of small dimeric molecules around 100 residues in length. The original members of this family, S100A and S100B, were initially identified in bovine brain. Other S100 proteins have also been identified, such as S100P from human placenta and S100L from bovine lung. The S100 family consists of at least 10 members which show a cell-type-specific expression pattern. Most S100 proteins are believed to be involved in Ca2+ signaling pathways. S100P consists of 95 amino acids and shares about 50% sequence identity with S100A and S100B, while S100L has been shown to possess 43-47% homology to S100A and S100B. S100B is expressed in the nervous system by glial cells, such as astrocyte, oligodendroglial, ependymal, and microglial cells. The dimeric form of S100B has been implicated in neuronal survival, neurite extension, and astrocyte growth. In addition, S100B expression is induced by stimulations that activate p53, and S100B activity may be involved in PKC-dependent p53 nuclear translocation in early G1 phase. Thus, S100B plays a role in calcium signaling pathways that regulate cell differentiation and cell cycle progression.

Molecular Weight: 8 kDa

Pathways: Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Toll-Like Receptors Cascades, Regulation of long-term Neuronal Synaptic Plasticity, S100 Proteins