SLU7 antibody (AA 457-570)

Catalog No. ABIN968890

This Mouse Monoclonal antibody specifically detects SLU7 in WB and IF. It exhibits reactivity toward Human, Mouse, Rat, Dog and Chicken and has been mentioned in 2+ publications.

Quick Overview for SLU7 antibody (AA 457-570) (ABIN968890)

Target: SLU7 (SLU7 Splicing Factor Homolog (SLU7))

Reactivity: Human, Mouse, Rat, Dog, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This SLU7 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 35-hSlu7

Product Details anti-SLU7 Antibody

Binding Specificity: AA 457-570

Cross-Reactivity: Dog (Canine), Rat (Rattus), Mouse (Murine)

No Cross-Reactivity: Chicken

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human hSlu7 aa. 457-570

Isotype: IgG2b

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-SLU7 antibody (ABIN968890)

Chua, Reed: "The RNA splicing factor hSlu7 is required for correct 3' splice-site choice." in: Nature, Vol. 402, Issue 6758, pp. 207-10, (2000) (PubMed).

Chua, Reed: "Human step II splicing factor hSlu7 functions in restructuring the spliceosome between the catalytic steps of splicing." in: Genes & development, Vol. 13, Issue 7, pp. 841-50, (1999) (PubMed).

Target Details for SLU7

Target: SLU7 (SLU7 Splicing Factor Homolog (SLU7))

Alternative Name: hSlu7

Target Type: Influenza Protein

Background: Splicing, the removal of introns from pre-mRNA, is mediated by spliceosomal complexes and occurs in two distinct catalytic steps. The first step involves cleavage of the 5' exon and the production of a lariat intermediate. In the second step, the 3' splice site is cleaved and the exons are fused with concomitant release of the intron lariat. The spliceosome contains multiple snRNPs and a number of non-snRNP splicing factors. Four yeast proteins (Prp16p, Prp17p, Prp18p, and Slu7p) function exclusively in the second catalytic step. Human homologs have been identified for Prp16p (hPrp16), Prp17p (hPrp17), Prp18p (hPrp18) and Slu7 (hSlu7). hSlu7 contains a zinc knuckle motif similar to the yeast Slu7. This domain is present in retroviral nucleocapsid proteins and in several splicing factors. hSlu7 associates with the spliceosome late in the splicing pathway during recognition of the 3' splice site. During step II of 3' splicing, hSlu7 tightly binds to exon 1 in the spliceosome and helps specify attack on the correct adenine-guanine dinucleotide, located 18 to 40 nucleotides downstream of the branch site.

Molecular Weight: 80 kDa

Pathways: Ribonucleoprotein Complex Subunit Organization, SARS-CoV-2 Protein Interactome