BMI1 antibody

Catalog No. ABIN968985

This anti-BMI1 antibody is a Mouse Monoclonal antibody detecting BMI1 in IHC, ELISA, ICC and FACS. Suitable for Human. This Primary Antibody has been cited in 2+ publications.

Quick Overview for BMI1 antibody (ABIN968985)

Target: BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This BMI1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 3E3

Product Details anti-BMI1 Antibody

Purpose: BMI1 Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human BMI1 expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-BMI1 antibody (ABIN968985)

Edwards, Witherspoon, Wang, Afrasiabi, Pham, Birnbaumer, Lipkin: "Epigenetic repression of DNA mismatch repair by inflammation and hypoxia in inflammatory bowel disease-associated colorectal cancer." in: Cancer research, Vol. 69, Issue 16, pp. 6423-9, (2009) (PubMed).

Bhattacharya, Nicoloso, Arvizo, Wang, Cortez, Rossi, Calin, Mukherjee: "MiR-15a and MiR-16 control Bmi-1 expression in ovarian cancer." in: Cancer research, Vol. 69, Issue 23, pp. 9090-5, (2009) (PubMed).

Target Details for BMI1

Target: BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))

Alternative Name: BMI1

Background: Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones, it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.

Molecular Weight: 37 kDa

Gene ID: 648

UniProt: P35226

Pathways: Cell Division Cycle, Autophagy