BTK antibody

Catalog No. ABIN968992

This Mouse Monoclonal antibody specifically detects BTK in WB, ELISA, IHC and ICC. It exhibits reactivity toward Human and Monkey.

Quick Overview for BTK antibody (ABIN968992)

Target: BTK (Bruton Agammaglobulinemia tyrosine Kinase (BTK))

Reactivity: Human, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This BTK antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Clone: 7F12H4

Product Details anti-BTK Antibody

Purpose: BTK Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of BTK expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Target Details for BTK

Target: BTK (Bruton Agammaglobulinemia tyrosine Kinase (BTK))

Alternative Name: BTK

Background: Brutons tyrosine kinase (BTK) is a member of the BTK/Tec family of cytoplasmic tyrosine kinases.All members of the family contain SH3 and SH2 domains and, with the exception of Txk and Dsrc28C, also contain a pleckstrin homology (PH) and a Tec homology (TH) domain in their amino termini.BTK plays an important role in B cell development. Activation of B cells by various ligands is accompanied by BTK membrane translocation mediated by its PH domain binding to phosphatidylinositol-3,4,5-trisphosphate. The membrane located BTK is active and associated with transient phosphorylation of two tyrosine residues, Tyr551 and Tyr223. Tyr551 in the activation loop is transphosphorylated by the Src family tyrosine kinase, leading to autophosphorylation at Tyr223 within the SH3 domain, which is necessary for full activation.

Molecular Weight: 77 kDa

Gene ID: 695

UniProt: Q06187

Pathways: Fc-epsilon Receptor Signaling Pathway, Hormone Transport, Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, BCR Signaling