Cyclin B1 antibody

Catalog No. ABIN969005

The Mouse Monoclonal anti-Cyclin B1 antibody has been validated for WB, IHC and ELISA. It is suitable to detect Cyclin B1 in samples from Human. There are 2+ publications available.

Quick Overview for Cyclin B1 antibody (ABIN969005)

Target: Cyclin B1 (CCNB1)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cyclin B1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: 1B10

Product Details anti-Cyclin B1 Antibody

Purpose: CCNB1 Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human CCNB1 expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes: ELISA: 1/10000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-Cyclin B1 antibody (ABIN969005)

Zhang, Li, Guo, Cao, Zhao, Jiang, Ma, Yi, Li, Jiang, Wu, Wang, Si: "DH166, a beta-carboline derivative, inhibits the kinase activity of PLK1." in: Cancer biology & therapy, Vol. 8, Issue 24, pp. 2374-83, (2010) (PubMed).

Okayama, Kumamoto, Saitou, Hayase, Kofunato, Sato, Miyamoto, Nakamura, Ohki, Sekikawa, Takenoshita: "CD44v6, MMP-7 and nuclear Cdx2 are significant biomarkers for prediction of lymph node metastasis in primary gastric cancer." in: Oncology reports, Vol. 22, Issue 4, pp. 745-55, (2009) (PubMed).

Target Details for Cyclin B1

Target: Cyclin B1 (CCNB1)

Alternative Name: CCNB1

Background: The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. (provided by RefSeq) It has higher expression in tumor tissues .

Molecular Weight: 60 kDa

Gene ID: 891

UniProt: P14635

Pathways: Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase