GPI antibody

Catalog No. ABIN969176

The Mouse Monoclonal anti-GPI antibody has been validated for ELISA, IHC and ICC. It is suitable to detect GPI in samples from Human. There are 3+ publications available.

Quick Overview for GPI antibody (ABIN969176)

Target: GPI (Glucose-6-Phosphate Isomerase (GPI))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GPI antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Clone: 1B7D7

Product Details anti-GPI Antibody

Purpose: Glucose-6-phosphate isomerase Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human GPI expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-GPI antibody (ABIN969176)

Schaller, Stohl, Benoit, Tan, Johansen, Ditzel: "Patients with inflammatory arthritic diseases harbor elevated serum and synovial fluid levels of free and immune-complexed glucose-6-phosphate isomerase (G6PI)." in: Biochemical and biophysical research communications, Vol. 349, Issue 2, pp. 838-45, (2006) (PubMed).

Haga, Komazaki, Funasaka, Hashimoto, Yokoyama, Watanabe, Raz, Nagase: "AMF/G6PI induces differentiation of leukemic cells via an unknown receptor that differs from gp78." in: Leukemia & lymphoma, Vol. 47, Issue 10, pp. 2234-43, (2006) (PubMed).

Muraki, Matsumoto, Chino, Hayashi, Suzuki, Goto, Ito, Murata, Tsutsumi, Sumida: "Glucose-6-phosphate isomerase variants play a key role in the generation of anti-GPI antibodies: possible mechanism of autoantibody production." in: Biochemical and biophysical research communications, Vol. 323, Issue 2, pp. 518-22, (2004) (PubMed).

Target Details for GPI

Target: GPI (Glucose-6-Phosphate Isomerase (GPI))

Alternative Name: Glucose-6-phosphate isomerase

Background: Glucose-6-phosphate isomerase, or phosphoglucose isomerase, also known as GPI. It belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways and it is an enzyme that catalyzes the conversion of glucose-6-phosphate into fructose 6-phosphate in the second step of glycolysis. The protein functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in GPI are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.

Molecular Weight: 63 kDa

Gene ID: 2821

UniProt: P06744