SKP1 antibody

Catalog No. ABIN969397

This Mouse Monoclonal antibody specifically detects SKP1 in WB, ELISA, IHC, ICC and FACS. It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for SKP1 antibody (ABIN969397)

Target: SKP1 (S-Phase Kinase-Associated Protein 1 (SKP1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SKP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 4E11

Product Details anti-SKP1 Antibody

Purpose: SKP1 Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human SKP1 expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-SKP1 antibody (ABIN969397)

Werden, Lanchbury, Shattuck, Neff, Dufford, McFadden: "The myxoma virus m-t5 ankyrin repeat host range protein is a novel adaptor that coordinately links the cellular signaling pathways mediated by Akt and Skp1 in virus-infected cells." in: Journal of virology, Vol. 83, Issue 23, pp. 12068-83, (2009) (PubMed).

Target Details for SKP1

Target: SKP1 (S-Phase Kinase-Associated Protein 1 (SKP1))

Alternative Name: SKP1

Background: This gene encodes a component of SCF complexes, which are composed of this protein, cullin 1, a ring-box protein, and one member of the F-box family of proteins. This protein binds directly to the F-box motif found in F-box proteins. SCF complexes are involved in the regulated ubiquitination of specific protein substrates, which targets them for degradation by the proteosome. Specific F-box proteins recognize different target protein(s), and many specific SCF substrates have been identified including regulators of cell cycle progression and development. Studies have also characterized the protein as an RNA polymerase II elongation factor. Alternative splicing of this gene results in two transcript variants. A related pseudogene has been identified on chromosome 7.

Molecular Weight: 19 kDa

UniProt: P63208

Pathways: Cell Division Cycle, Hedgehog Signaling, Mitotic G1-G1/S Phases