TRIM5 antibody

Catalog No. ABIN969443

Product Details anti-TRIM5 Antibody

Target: TRIM5 (Tripartite Motif Containing 5 (TRIM5))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TRIM5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purpose: Trim5a Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human trim5 alpha expressed in E. Coli.

Clone: 3B11H2

Isotype: IgG1

Application Details

Application Notes: ELISA: 1/10000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-TRIM5 antibody (ABIN969443)

Song, Javanbakht, Perron, Park, Stremlau, Sodroski: "Retrovirus restriction by TRIM5alpha variants from Old World and New World primates." in: Journal of virology, Vol. 79, Issue 7, pp. 3930-7, (2005) (PubMed).

Keckesova, Ylinen, Towers: "The human and African green monkey TRIM5alpha genes encode Ref1 and Lv1 retroviral restriction factor activities." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 29, pp. 10780-5, (2004) (PubMed).

Stremlau, Owens, Perron, Kiessling, Autissier, Sodroski: "The cytoplasmic body component TRIM5alpha restricts HIV-1 infection in Old World monkeys." in: Nature, Vol. 427, Issue 6977, pp. 848-53, (2004) (PubMed).

Target Details for TRIM5

Target: TRIM5 (Tripartite Motif Containing 5 (TRIM5))

Alternative Name: Trim5a (TRIM5 Products)

Background: TRIM5-alpha is a protein that is found in the cells of many mammals and fends of various retrovirus infections. It protects monkeys from infection with HIV-1, and humans from infection with some other viruses. If a retrovirus has entered a cell, it needs to shed its capsid in order to reversely transcribe its genes, so that they can be expressed by the host cell. It is believed that TRIM5 alpha, which is present in the cytoplasm, somehow recognizes the capsid and blocks its shedding, thereby stopping the virus in its tracks. It thus represents an intracellular defense completely separate from the rest of the body's immune system.

Gene ID: 85363

UniProt: Q9C035

Pathways: Activation of Innate immune Response