TRIM5 antibody (truncated)

Catalog No. ABIN2464117

Product Details anti-TRIM5 Antibody

Target: TRIM5 (Tripartite Motif Containing 5 (TRIM5))

Binding Specificity: truncated

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TRIM5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Immunogen: Ni-NTA purified truncated recombinant human trim5 alpha expressed in E. Coli strain BL21 (DE3).

Clone: 3B11H2

Isotype: IgG1

Application Details

Application Notes: Western Blot:Dilution 1:1,000 - 1:5,000
IHC(P):Dilution 1:100 - 1:400
IHC(F):Dilution 1:200 - 1:400
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Storage: -20 °C

Storage Comment: Trim5? monoclonal antibody can be stored at -20 °C, stable for one year.

Target Details for TRIM5

Target: TRIM5 (Tripartite Motif Containing 5 (TRIM5))

Alternative Name: Trim5a (TRIM5 Products)

Synonyms: TRIM5 antibody, RNF88 antibody, TRIM5alpha antibody, EG667823 antibody, Gm8833 antibody, RGD1304579 antibody, tripartite motif containing 5 antibody, tripartite motif-containing 5 antibody, TRIM5 antibody, Trim5 antibody

Background: TRIM5-alpha is a protein that is found in the cells of many mammals and fends of various retrovirus infections. It protects monkeys from infection with HIV-1, and humans from infection with some other viruses. If a retrovirus has entered a cell, it needs to shed its capsid in order to reversely transcribe its genes, so that they can be expressed by the host cell. It is believed that TRIM5alpha, which is present in the cytoplasm, somehow recognizes the capsid and blocks its shedding, thereby stopping the virus in its tracks. It thus represents an intracellular defense completely separate from the rest of the body's immune system.

Gene ID: 85364

UniProt: Q9NUD5

Pathways: Activation of Innate immune Response