MTDH antibody

Catalog No. ABIN969496

This Mouse Monoclonal antibody specifically detects MTDH in WB, IHC, ELISA and FACS. It exhibits reactivity toward Human and has been mentioned in 2+ publications.

Quick Overview for MTDH antibody (ABIN969496)

Target: MTDH (Metadherin (MTDH))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MTDH antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Clone: 2F11C3

Product Details anti-MTDH Antibody

Purpose: Metadherin Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human Metadherin expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-MTDH antibody (ABIN969496)

Britt, Yang, Yang, Flanagan, Callanan, Lim, Lin, Hixson: "Identification of a novel protein, LYRIC, localized to tight junctions of polarized epithelial cells." in: Experimental cell research, Vol. 300, Issue 1, pp. 134-48, (2004) (PubMed).

Brown, Ruoslahti: "Metadherin, a cell surface protein in breast tumors that mediates lung metastasis." in: Cancer cell, Vol. 5, Issue 4, pp. 365-74, (2004) (PubMed).

Target Details for MTDH

Target: MTDH (Metadherin (MTDH))

Alternative Name: Metadherin

Background: Metadherin (Metastasis adhesion protein), also known as MTDH, LYsine-RIch CEACAM1 co-isolated (LYRIC), is a novel protein that localizes with the tight junction proteins ZO-1 and occludin in polarized epithelial cells. At the tight junction, it acts not as a structural component, but is rather recruited during the maturation of the tight junction complex. Metadherin is overexpressed in breast cancer tissue and breast tumor xenografts, while much lower levels are expressed in normal breast tissue. Metadherin binds to lung vasculature, one of the four common sites of breast cancer metastasis, through a C-terminal segment in the extracellular domain, blocking this lung-homing domain with antibodies or inhibiting metadherin with siRNA has been reported to inhibit breast cancer metastasis.

Molecular Weight: 64 kDa

UniProt: Q86UE4

Pathways: Cellular Response to Molecule of Bacterial Origin, Cell-Cell Junction Organization